Determination of Mulberroside A in Ramulus Mori by HPLC

FANG Jing1; GAO Fei2; WU Hong-wei1; LI Shao-jing1; XU Hai-yu1; YANG Hong-jun1; YANG Lan1; FU Mei-hong1; LIU Feng3

doi:10.13422/j.cnki.syfjx.2013.05.048

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Determination of Mulberroside A in Ramulus Mori by HPLC

更新时间：2024-09-23

- Determination of Mulberroside A in Ramulus Mori by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 5, Pages: 80-82(2013)
- 作者机构：
  
  1. 中国中医科学院中药研究所
  2. 首都医科大学中医药学院
  3. 陕西步长制药有限公司
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.05.048
  CLC： R284.1
- Published：2013
- 稿件说明：
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[1]方婧,高霏,吴宏伟,李韶菁,许海玉,杨洪军,杨岚,付梅红,刘峰.桑枝中桑皮苷A的含量测定[J].中国实验方剂学杂志,2013,19(05):80-82.

FANG Jing1, GAO Fei2, WU Hong-wei1, et al. Determination of Mulberroside A in Ramulus Mori by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 80-82.
[1]方婧,高霏,吴宏伟,李韶菁,许海玉,杨洪军,杨岚,付梅红,刘峰.桑枝中桑皮苷A的含量测定[J].中国实验方剂学杂志,2013,19(05):80-82. DOI： 10.13422/j.cnki.syfjx.2013.05.048.

FANG Jing1, GAO Fei2, WU Hong-wei1, et al. Determination of Mulberroside A in Ramulus Mori by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 80-82. DOI： 10.13422/j.cnki.syfjx.2013.05.048.

摘要

目的:建立桑枝中桑皮苷A的含量测定方法。方法:Dikma Diamonsil C18色谱柱（4.6 mm×250 mm

5μm）

流动相为甲醇-水（15∶85）

检测波长324 nm

柱温30℃

流速1 mL.min-1。结果:桑皮苷A在10.34～206.8μg呈良好线性关系（r=0.999 9）

平均回收率99.8%;11批桑枝药材中桑皮苷A含量在0.10%～1.69%。结论:此法准确、简便、快速

可用于检测桑枝中桑皮苷A的含量。

Abstract

Objective:To establish a method for the determination of mulberroside A in Ramulus Mori by HPLC.Method: Dikma Diamonsil C18 column（4.6 mm×250 mm

5 μm） was used.The mobile phase consisted of methanol and water（15∶85）.The temperature of column was maintained at 30 ℃.The flow rate was 1.0 mL ·min-1 and the detection wavelength was set at 324 nm.Result: The calibration curve was in good linearity within the range from 10.34 to 206.8μg（r=0.999 9）.The average recovery was 99.8%.The content of mulberroside A in Ramulus Mori in defferent areas was 0.10%-1.69%.Conclusion: The method is simple

rapid as well as precise and reliable

and can be used for the determination of mulberroside A in Ramulus Mori.

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Related Author

FANG Jing

GAO Fei

WU Hong-wei

LI Shao-jing

XU Hai-yu

YANG Hong-jun

YANG Lan

FU Mei-hong

Related Institution

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

Jilin Ginseng Academy，Changchun University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

School of Pharmacy， Henan University of Chinese Medicine

Sunflower Pharmaceutical Group（Xiangyang） Longzhong Co. Ltd.，Xiangyang

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