Objective:To establish a UPLC method for simultaneous determination of saikosaponins a

c

d in Bupleurum chinense.Method: Saikosaponins a

c

d in B.chinense were simultaneously determined by UPLC with Waters ACQUITY UPLC BEH C18（2.1 mm ×50 mm

1.7 μm） column.With a gradient program of acetonitrile-water at the flow rate of 0.4 mL·min-1

use an evaporative light-scattering detector（UPLC-ELSD） as the detector.The drift tube temperature was set at 40 ℃;the gas flow rate was 2.5 L·min-1.Result: Saikosaponins a

c

d in B.chinense were separated clearly and respectively in 6 min.The linear ranges of Saikosaponins a

c

d were 0.043 6-0.348 8

0.046 8-0.374 4 and 0.049 2-0.393 6 μg respectively.The RSD of recovery

precision and reproducibility were all less than 3.0%.Conclusion: The method is rapid

simple

reliable and accurate

has been successfully used to the quantification of saikosaponins a

c

d in B.chinense.