Determination of Catechin in Eucommia ulmoids by RP-HPLC

JIA Zhi-ruo1; LI Bing1; LI Yao-hua1; ZHEN Han-shen1; LU Cheng-sheng1

doi:10.13422/j.cnki.syfjx.2013.05.057

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Determination of Catechin in Eucommia ulmoids by RP-HPLC

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- Determination of Catechin in Eucommia ulmoids by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 5, Pages: 117-119(2013)
- 作者机构：
  
  1. 广西中医药大学
  2. 成都中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.05.057
  CLC： R284.1
- Published：2013
- 稿件说明：
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[1]贾智若,李兵,李耀华,甄汉深,卢澄生.反相高效液相色谱法测定杜仲中儿茶素的含量[J].中国实验方剂学杂志,2013,19(05):117-119.

JIA Zhi-ruo1, LI Bing1, LI Yao-hua1, et al. Determination of Catechin in Eucommia ulmoids by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 117-119.
[1]贾智若,李兵,李耀华,甄汉深,卢澄生.反相高效液相色谱法测定杜仲中儿茶素的含量[J].中国实验方剂学杂志,2013,19(05):117-119. DOI： 10.13422/j.cnki.syfjx.2013.05.057.

JIA Zhi-ruo1, LI Bing1, LI Yao-hua1, et al. Determination of Catechin in Eucommia ulmoids by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 117-119. DOI： 10.13422/j.cnki.syfjx.2013.05.057.

摘要

目的:建立测定杜仲中儿茶素含量的高效液相色谱方法。方法:设计正交试验对样品的前处理过程进行优化

采用反相高效液相色谱法测定杜仲中儿茶素含量

色谱柱为Agilent HC-C18（4.6 mm×250 mm

5μm）;流动相乙腈-0.4%磷酸溶液（13∶87）;流速1.0 mL.min-1

检测波长280 nm。结果:儿茶素在9.620～194.0 mg.L-1线性关系良好（r=0.999 3）

平均加样回收率为97.38%

RSD 2.1%。结论:该法简便快速、准确性和重复性好

可用于杜仲中儿茶素的含量测定。

Abstract

Objective:To establish a HPLC method for the determination of catechin in Eucommia ulmoides.Method: The orthogonal test was used to optimize the pretreatment process.RP-HPLC was adopted

on a column of Agilent HC-C18（4.6 mm×250 mm

5 μm）

using the mobile phase of acetonitrile-0.4% phosphoric acid solution（13∶87）

at a flow rate of 1.0 mL·min-1;the detection wavelength was set at 280 nm.Result: Catechin was linear in the range of 9.620-194.0 μg·mL-1（r=0.999 3）.The average recovery of catechin was 97.38% with RSD 2.1%.Conclusion: The method is simple

rapid and reliable with good reproduciblity

which can be used for the content determination of catechin in E.ulmoides.

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Related Author

JIA Zhi-ruo

LI Bing

LI Yao-hua

ZHEN Han-shen

LU Cheng-sheng

GENG Dan

MA Wen-Fang

ZHEN Han-shen

Related Institution

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

Jilin Ginseng Academy，Changchun University of Chinese Medicine

Sunflower Pharmaceutical Group（Xiangyang） Longzhong Co. Ltd.，Xiangyang

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijng

Shanxi University of Chinese Medicine

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