Determination of Three Kind Ingredients from Yin Chen by RP-HPLC

LI Li; SUN Yan-tao; WANG Bing

doi:10.13422/j.cnki.syfjx.2013.05.061

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Determination of Three Kind Ingredients from Yin Chen by RP-HPLC

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- Determination of Three Kind Ingredients from Yin Chen by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 5, Pages: 136-138(2013)
- 作者机构：
  
  辽宁中医药大学药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.05.061
  CLC： R284.1
- Published：2013
- 稿件说明：
移动端阅览
[1]李莉,孙艳涛,王冰.RP-HPLC同时测定茵陈中3种有效成分含量[J].中国实验方剂学杂志,2013,19(05):136-138.

LI Li, SUN Yan-tao, WANG Bing. Determination of Three Kind Ingredients from Yin Chen by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 136-138.
[1]李莉,孙艳涛,王冰.RP-HPLC同时测定茵陈中3种有效成分含量[J].中国实验方剂学杂志,2013,19(05):136-138. DOI： 10.13422/j.cnki.syfjx.2013.05.061.

LI Li, SUN Yan-tao, WANG Bing. Determination of Three Kind Ingredients from Yin Chen by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 136-138. DOI： 10.13422/j.cnki.syfjx.2013.05.061.

摘要

目的:采用梯度洗脱法测定不同不同商品地茵陈中金丝桃苷、绿原酸和齐墩果酸的含量。方法:采用Diamonsil C18（4.6 mm×200 mm

5μm）色谱柱

以乙腈-0.4%磷酸水溶液为流动相

流速1.0 mL.min-1

检测波长235 nm进行梯度洗脱。结果:不同商品地茵陈中3种成分的含量差异明显

金丝桃苷含量2.93～15.58 mg.g-1

绿原酸5.858～47.73 mg.g-1和齐墩果酸1.89～4.98 mg.g-1。结论:方法快速可靠

丰富茵陈质量标定方法。

Abstract

Objective:To determine the content of hypericin

chlorogenic acid and oleanolic acid from Yin Chen of different commodity by gradient elution.Method: The Diamonsil C18column（4.6 mm×200 mm

5 μm） was used with acetonitrile-0.4% phosphoric acid as mobile phase;the flow rate was 1.0 mL·min-1

and detected at the wavelength of 235 nm.Result: The content of active ingredients from Yin Chen was different.The content of hypericin

chlorogenic acid and oleanolic acid was 2.93-15.58

5.858-47.73

1.89-4.98 mg·g-1 respectively.Conclusion: The method can expend the content determination of the active ingredients

and help to establish the quality standards of Yin Chen.

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Related Author

LI Li

SUN Yan-tao

WANG Bing

GUO Ju-liang

LI Xin-xia

ZHI Ling

LIN Xiu-lian

SONG Fen-yun

Related Institution

Institute of Traditional Chinese Medicine（TCM），Shanghai University of TCM

Yanling DU Renkang Biotechnology Co. Ltd.

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Henan University of Chinese Medicine

Engineering Technology Research Center for Comprehensive Development and Utilization of Authentic Medicinal Materials in Henan Province， Henan University of Chinese Medicine

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