Determination of Nvzhenoside in Liuwei Wuling Tablet by HPLC

LIU Hong-ming1; LIU Yu-xia2; XU Nan-nan3

doi:10.13422/j.cnki.syfjx.2013.05.065

您当前的位置：

首页 >

文章列表页 >

Determination of Nvzhenoside in Liuwei Wuling Tablet by HPLC

更新时间：2024-09-23

- Determination of Nvzhenoside in Liuwei Wuling Tablet by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 5, Pages: 147-149(2013)
- 作者机构：
  
  1. 山东省淄博市药品检验所
  2. 张店区中医院
  3. 山东大学药学院
  4. 淄博市食品药品监督管理局
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.05.065
  CLC： R286.0
- Published：2013
- 稿件说明：
移动端阅览
[1]刘宏明,刘昱霞,徐楠楠.HPLC测定六味五灵片中特女贞苷的含量[J].中国实验方剂学杂志,2013,19(05):147-149.

LIU Hong-ming1, LIU Yu-xia2, XU Nan-nan3. Determination of Nvzhenoside in Liuwei Wuling Tablet by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 147-149.
[1]刘宏明,刘昱霞,徐楠楠.HPLC测定六味五灵片中特女贞苷的含量[J].中国实验方剂学杂志,2013,19(05):147-149. DOI： 10.13422/j.cnki.syfjx.2013.05.065.

LIU Hong-ming1, LIU Yu-xia2, XU Nan-nan3. Determination of Nvzhenoside in Liuwei Wuling Tablet by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 147-149. DOI： 10.13422/j.cnki.syfjx.2013.05.065.

摘要

目的:采用HPLC法测定六味五灵片中特女贞苷的含量。方法采用Agilent ZORBAX SB（250 mm×4.6 mm

5μm）色谱柱

流动相为甲醇-水（30∶70）

检测波长224 nm

流速1.0 mL.min-1

柱温35℃。结果:特女贞苷在0.246 6～3.699μg范围内线性关系良好

线性关系方程为:Y=1 233 200X+2.476 4×104

r=0.999 9。特女贞苷平均回收率为99.96%

RSD 0.58%。结论:方法灵敏、准确、专属性强

重复性好

可用于测定六味五灵片中特女贞苷的含量。

Abstract

Objective:To establish the method for determining nvzhenoside in Liuwei Wuling tablet by HPLC.Method: Nvzhenoside was analyzed by HPLC.The optimal conditions of separation and detection were achieved on a Agilent ZORBAX SB（4.6 mm×250 mm

5 μm） by isocratic elution with the mobile phase of methanol-water（30∶ 70） at the flow rate of 1.0 mL·min-1.The detective wavelength of deoxyschizandrin was set at 224 nm.Result: The regression equation of nvzhenoside was Y=1 233 200X+2.476 4×104（r=0.999 9）.So the linearity was acceptable.The average recovery of nvzhenoside was 99.96%（RSD 0.58%）.Conclusion: This method was simple

rapid and feasible for the quantification of nvzhenoside in Liuwei Wuling tablet by HPLC.

关键词

Keywords

references

Views

193

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Determination of Nvzhenoside in Liuwei Wuling Tablet by HPLC

Determination of Glycyrrhizin in Weiwanshu Granule by HPLC

Determination of Icariin in Syrup by HPLC

双黄连含片中黄芩甙、绿原酸及连翘甙的含量测定

Determination of Puerarin in Gegen Qinlian Weiwan by HPLC

Related Author

LIU Hong-ming

LIU Yu-xia

XU Nan-nan

SONG Hong-jie

QUAN Shan-cong

HU Jin-hong

ZHU Bing-hui1

LIANG Yi-ying1

Related Institution

广西中医药大学赛恩斯新医药学院,广西中医药大学,广西中医药大学赛恩斯新医药学院

成都中医药大学,九江学院,重庆市中药研究院重庆市中药资源重点实验室,重庆市中药研究院重庆市中药资源重点实验室,重庆市中药研究院重庆市中药资源重点实验室

Dongcheng District Institute for Drug Control

College of Pharmaceutical Science,Dali University,Dali

Shanxi Buchang Pharmaceutical Co.Ltd

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰