Effect of Xiaoyong Kuidekang on Expressions of Growth Factors and Apoptosis Related Factors in Rat Ulcer Model

SUN Yun-feng; WANG Hao; JIANG Ning; QU Yi; LIN Shu-ru; CAI Li-ping; ZHENG Hong-xin; ZHOU Xue-wen

doi:10.13422/j.cnki.syfjx.2013.05.073

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Effect of Xiaoyong Kuidekang on Expressions of Growth Factors and Apoptosis Related Factors in Rat Ulcer Model

更新时间：2024-09-23

- Effect of Xiaoyong Kuidekang on Expressions of Growth Factors and Apoptosis Related Factors in Rat Ulcer Model
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 5, Pages: 186-190(2013)
- 作者机构：
  
  辽宁中医药大学基础医学院中医分子生物学实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.05.073
  CLC： R285.5
- Published：2013
- 稿件说明：
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[1]孙云峰,王浩,蒋宁,曲怡,林庶茹,才丽平,郑洪新,周学文.消痈溃得康对胃溃疡模型大鼠生长因子和凋亡相关因子的影响[J].中国实验方剂学杂志,2013,19(05):186-190.

SUN Yun-feng, WANG Hao, JIANG Ning, et al. Effect of Xiaoyong Kuidekang on Expressions of Growth Factors and Apoptosis Related Factors in Rat Ulcer Model[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 186-190.
[1]孙云峰,王浩,蒋宁,曲怡,林庶茹,才丽平,郑洪新,周学文.消痈溃得康对胃溃疡模型大鼠生长因子和凋亡相关因子的影响[J].中国实验方剂学杂志,2013,19(05):186-190. DOI： 10.13422/j.cnki.syfjx.2013.05.073.

SUN Yun-feng, WANG Hao, JIANG Ning, et al. Effect of Xiaoyong Kuidekang on Expressions of Growth Factors and Apoptosis Related Factors in Rat Ulcer Model[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 186-190. DOI： 10.13422/j.cnki.syfjx.2013.05.073.

摘要

目的:探讨中药消痈溃得康对乙酸所诱导的大鼠胃溃疡模型治疗作用的分子机制。方法:将40只大鼠随机分为正常组、假手术组、模型组、消痈溃得康组和奥美拉唑组

每组8只。采取胃黏膜局部投与乙酸方法制备胃溃疡模型

并于造模后第2天开始给药

消痈溃得康组按照4 g.kg-1剂量灌胃给予消痈溃得康颗粒

奥美拉唑组按照1.25 g.kg-1剂量给予奥美拉唑。假手术组、模型组给予纯水

每日一次

共灌胃10 d。10 d后取血清及胃组织进行检测。采用酶联免疫吸附（ELISA）方法检测血清中血管内皮生长因子（VEGF）、表皮生长因子（EGF）及与凋亡相关的因子Bcl-2、C-myc的含量。取溃疡局部胃组织

Trozol法提取RNA

反转录为cDNA后采用实时荧光定量PCR法检测胃组织中EGFmRNA表达。结果:假手术组与正常组相比大鼠血清中EGF

VEGF

Bcl-2

C-myc含量没有显著差异;模型组中EGF

Bcl-2含量低于正常组（P<0.05）

VEGF

C-myc的含量高于正常组（P<0.05）;消痈溃得康组、奥美拉唑组大鼠血清中EGF

VEGF

Bcl-2含量高于模型组（P<0.05）;消痈溃得康组、奥美拉唑组大鼠血清中C-myc含量低于模型组（P<0.05）。荧光定量PCR检测EGFmRNA表达结果显示:假手术组与正常组相比没有差异

中药消痈溃得康治疗组大鼠胃组织EGF mRNA表达水平明显高于模型组（P<0.05）。结论:中药消痈溃得康可以提高大鼠血清中VEGF

EGF等生长因子的表达

促进胃黏膜上皮及血管内皮细胞的生长

促进愈合

提高Bcl-2水平

抑制凋亡。同时降低C-myc水平

减少了癌变发生几率。

Abstract

Objective:To study the molecular mechanism of the treatment in which Xiaoyong Kuidekang would be applied to a model of gastric ulceration in rats.Method:Rats were divided into 5 groups randomly:normal group

sham group

ulcer model group

Xiaoyong Kuidekang group and Omeprazole group.Eight rats in each group.Acetic acid was applied to a certain part of the gastric mucosa to establish the ulcer model.The Xiaoyong Kuidekang treatment group had been administrated with drugs 4 g·kg-1and the Omeprazole group had been administrated with drugs 1.25 g·kg-1 since the second day after the operation.after 10 days The stomach and the serum was taken.The healing process of gastric ulcers was observed and the areas was measured.The content of EGF

VEGF

Bcl-2

and C-myc was measured by ELISA method.Total RNAs were extracted from the ulcer part of the gastric tissue by Trizol method

then the RNAs were transcribed reversely into cDNAs.The relative expression levels of EGF mRNA was measured by the QPCR assay.Result:There was no difference between the normal group and the sham operation group on levels of EGF

VEGF

C-myc and Bcl-2.the concentration of EGF

Bcl-2 in model group were lower than those in the normal group（P<0.05）and The concentration of VEGF

C-myc in model group were higher than those in the normal（P<0.05）.The concentration of EGF

VEGF or Bcl-2 in the Xiaoyong Kuidekang group and in the Omeprazole group were much higher than those in the ulcer model group（P<0.05）.The concentrations of C-myc in the Xiaoyong Kuidekang group and in the Omeprazole group were much lower than those in the ulcer model group（P<0.05）.The mered result of the the rat EGFmRNA expression through the QPCR method is revealed.The expression of EGFmRNA in the Xiaoyong Kuidekang group were much higher than that in the ulcer model group（P<0.05）.Conclusion:Xiaoyong Kuidekang can raise the expression of VEGF

EGF in rat serum

promote the growth of gastric epithelial and endothelial cell

promote healing

raise the level of Bcl-2

inhibit apoptosis.At the same time

reduce the level of C-myc

reducing the probability of occurrence of carcinogenesis.

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Related Author

SUN Yun-feng

Wang Hao

JIANG Ning

QU Yi

LIN Shu-ru

CAI Li-ping

ZHENG Hong-xin

ZHOU Xue-wen

Related Institution

The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine

First Affiliated Hospital of Liaoning University of TCM

Liaoning University of TCM Hospital Subsidiary Fourth

Liaoning University of Traditional Chinese Medicine（TCM）

School of Pharmacy， Academy of Chinese Medical Sciences， Henan University of Chinese Medicine

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