Effects of Xiaochaihu Decoction Containing Drug Serum on HepG-2 Cell

LI Ran1; LIU Li-ping1; MA Ji1; JIA Li-long2; LI Hai-bo1

doi:10.13422/j.cnki.syfjx.2013.05.078

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Effects of Xiaochaihu Decoction Containing Drug Serum on HepG-2 Cell

更新时间：2024-09-23

- Effects of Xiaochaihu Decoction Containing Drug Serum on HepG-2 Cell
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 5, Pages: 217-220(2013)
- 作者机构：
  
  1. 辽宁中医药大学基础医学院
  2. 辽宁中医药大学第一临床学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.05.078
  CLC： R285.5
- Published：2013
- 稿件说明：
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[1]李然,刘立萍,马骥,贾立龙,李海波.小柴胡汤含药血清对肝癌HepG-2细胞的影响[J].中国实验方剂学杂志,2013,19(05):217-220.

LI Ran1, LIU Li-ping1, MA Ji1, et al. Effects of Xiaochaihu Decoction Containing Drug Serum on HepG-2 Cell[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 217-220.
[1]李然,刘立萍,马骥,贾立龙,李海波.小柴胡汤含药血清对肝癌HepG-2细胞的影响[J].中国实验方剂学杂志,2013,19(05):217-220. DOI： 10.13422/j.cnki.syfjx.2013.05.078.

LI Ran1, LIU Li-ping1, MA Ji1, et al. Effects of Xiaochaihu Decoction Containing Drug Serum on HepG-2 Cell[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 217-220. DOI： 10.13422/j.cnki.syfjx.2013.05.078.

摘要

目的:探讨小柴胡汤含药血清抑制HepG-2肝癌细胞增殖的作用机制。方法:大鼠灌服小柴胡汤3.69 g.kg-1

空白组给予等量蒸馏水

1次/d

连续7 d

末次给药1 h后腹主动脉取血制备小柴胡汤含药血清。以HepG-2肝癌细胞为研究对象

实验分为10%空白血清组

10%

20%含药血清组。采用倒置显微镜观察含药血清对HepG-2细胞作用后的形态改变

噻唑蓝（MTT）法测定其对生长抑制的影响

流式细胞术分析细胞凋亡的情况

罗丹明123（Rh123）荧光染色观察细胞线粒体膜电位的变化。结果:与空白血清组比较

小柴胡汤含药血清能够抑制HepG-2肝癌细胞增殖

10%含药血清抑制细胞增殖具有时间依赖的特点;流式检测结果表明

10%小柴胡汤含药血清作用HepG-2肝癌细胞8 h后

凋亡率从6.78%上升至19.51%;Rh123荧光染色显示

细胞线粒体膜电位明显降低。结论:小柴胡汤含药血清能够抑制HepG-2肝癌细胞增殖

促进其凋亡

线粒体途径参与了该过程。

Abstract

Objective:To investigate the effects of Xiaochaihu decoction（XCHD） containing drug serum on the cell proliferation of hepatocarcinoma HepG-2 cell.Method: XCHD containing drug serum was prepared.The cells were separated into four groups: 10% control serum group

5% XCHD containing drug serum

10% XCHD containing drug serum and 20% XCHD containing drug serum.The HepG-2 cell morphologic changes were observed using the inverted microscope;HepG-2 cell viability was measured by MTT assay.Apoptosis was detected by flow cytometry assay.Mitochondrial membrane potential changes were measured using fluorescent rhodamine123.Result: Compared with the control group

XCHD containing drug serum could significantly inhibit the proliferation of HepG-2 cell and the inhibition effect of 10% XCHD containing drug serum exhibited time-dependent relationship;flow cytometry results showed that after being treated with 10% XCHD containing drug serum

the apoptosis rate of HepG-2 cell elevated from 6.78% to 19.51%;rhodamine123 staining revealed that the mitochondrial membrane potential was reduced.Conclusion: XCHD containing drug serum could inhibit the proliferation of hepatocarcinoma HepG-2 cells and promote the apoptosis rate.The main mechanism of XCHD inhibiting HepG-2 cell is related with mitochondrial function.

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Related Author

LI Ran

LIU Li-ping

MA Ji

JIA Li-long

LI Hai-bo

DU Mengge

LYU Bo

MENG Lingyu

Related Institution

Key Laboratory of Xinjiang Phytomedicine Resource and Utilization， Ministry of Education， School of Pharmacy， Shihezi University

First Affiliated Hospital， School of Medicine， Shihezi University

School of Chinese Materia Medica， Beijing University of Chinese Medicine

National Institutes for Food and Drug Control

Department of Oncology,Institute of Traditional Chinese Medicine

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