Rationality Evaluation of Different Extraction Technologies of Andrographis Herba

WANG Ya-qi; WU Zhen-feng; ZHENG Qin; HU Peng-yi; LAN Ji-ping; ZHANG Pu-zhao; YANG Ming

doi:10.13422/j.cnki.syfjx.2014100001

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Rationality Evaluation of Different Extraction Technologies of Andrographis Herba

更新时间：2024-01-04

- Rationality Evaluation of Different Extraction Technologies of Andrographis Herba
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 10, Pages: 1-5(2014)
- 作者机构：
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- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014100001
  CLC： R284.2
- Published：2014
- 稿件说明：
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WANG Ya-qi, WU Zhen-feng, ZHENG Qin, et al. Rationality Evaluation of Different Extraction Technologies of Andrographis Herba[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 1-5.
DOI：

WANG Ya-qi, WU Zhen-feng, ZHENG Qin, et al. Rationality Evaluation of Different Extraction Technologies of Andrographis Herba[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 1-5. DOI： 10.13422/j.cnki.syfjx.2014100001.

摘要

目的：比较不同穿心莲提取液中有效成分含量、指纹图谱和药效学活性。方法：采用减压提取法（VAE）、超声提取法（UAE）和传统提取法（HRE）制备提取液，利用HPLC对不同穿心莲提取物进行微观组分指纹图谱分析，流动相乙腈（A）-0.2%甲酸水溶液（B）梯度洗脱（0~20 min，10%~30%A；20~30 min，30%~35%A；30~40 min，35%~60%A；40~50 min，60%~85%A；50~60 min，85%~100%A），检测波长254 nm，结合DPPH自由基反应体系，考察不同工艺提取液的抗氧清除能力。结果：VAE对穿心莲药材中药效成分（穿心莲内酯、脱水穿心莲内酯）提取效率显著高于传统方法。3种提取液的指纹图谱均含有17个特征峰，VAE，HRE及UAE的相似度分别为0.957，0.911，0.905。清除DPPH自由基能力强弱顺序为VAE>UAE>HRE，当质量浓度为20 g·L-1时，DPPH清除率分别为44.85%，25.29%，16.58%。结论：穿心莲不同工艺提取液中药效成分不存在显著性差异。与传统提取法相比，VAE具有提取时间短、效率高、无污染等优点。VAE液对DPPH自由基清除效果优于HRE液和UAE液。

Abstract

Objective: To compare active ingredients contents

HPLC fingerprints and pharmacodynamics activities of different Andrographis Herba extracts. Method: Extracts were prepared by three different extraction technologies of vacuum-assist extraction(VAE)

ultrasound-assist extraction(UAE) and heating reflux extraction(HRE)

extraction efficiencies and micro-fingerprints analysis of extracts were investigated by HPLC

taking acetonitrile(A)-0.2% formic acid(B) (0-20 min

10%-30%A;20-30 min

30%-35%A;30-40 min

35%-60%A;40-50 min

60%-85%A;50-60 min

85%-100%A) as mobile phase for gradient elution

detection wavelength was set at 254 nm.Antioxidant activities of extracts were investigated by DPPH free radical reaction system. Result: Compared with traditional methods

extraction efficiencies of active ingredients(andrographolide and dehydroandrographolide) were higher by VAE.Analysis of HPLC fingerprint showed 17 main characteristic peaks in three different extracts

similarities of VAE

HRE and UAE were 0.957

0.911 and 0.905

respectively.DPPH free radical scavenging capacity was in the order of VAE>UAE>HRE

when the concentration of active ingredients was 20 g·L-1

DPPH clearance rates were 44.85%

25.29% and 16.58%

respectively. Conclusion: Active ingredients in different extracts of Andrographis Herba had no significant differences.Compared with traditional extracting methods

VAE had advantages

such as short extraction time

high efficiency

no pollution and so on.Scavenging effect of VAE solution on DPPH radical was better than HRE extract and UAE extract.

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