TAN Wei, DING Dong-mei, ZHANG Zhen-hai, et al. Determination of Apparent Solubility and Oil/water Partition Coefficient of Flavonoid Components from Epimedii Folium[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 5-7.
DOI:
TAN Wei, DING Dong-mei, ZHANG Zhen-hai, et al. Determination of Apparent Solubility and Oil/water Partition Coefficient of Flavonoid Components from Epimedii Folium[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 5-7. DOI: 10.13422/j.cnki.syfjx.2014100005.
Determination of Apparent Solubility and Oil/water Partition Coefficient of Flavonoid Components from Epimedii Folium
Objective: To determine apparent solubility and apparent oil/water partition coefficient of flavonoid components from Epimedii Folium
so as to lay foundation for overall solubility characterization of these components. Method: Taking flavonoid components from Epimedii Folium as model drug
HPLC was adopted to determine equilibrium solubilities and apparent oil/water partition coefficients(Papp) of epimedin A
B
C and icariin in different pH buffers by taking mobile phase of acetonitrile-water(25:75) and detection wavelength at 270 nm. Result: Overall equilibrium solubilities of flavonoid components from Epimedii Folium in different pH buffers were in order of epimedin B>epimedin A>epimedin C>icariin
oil/water partition coefficients were -1.437-3.147
overall trends of Papp were changed from small to large and then to small
to large at last. Conclusion: Water-soluble and fat-soluble of flavonoid components from Epimedii Folium were well with a similar overall trend