Optimization of Rapid Ultrasonic Extraction Process of Four Active Components from Salviae Miltiorrhizae Radix et Rhizoma by Uniform Design

GAO Shu-rui; ZHAO Zhi-gang; HOU Jun-ling; WANG Wen-quan; ZHU Jun-jie; WANG Li-hui; HAN Ya-nan

doi:10.13422/j.cnki.syfjx.2014100008

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Optimization of Rapid Ultrasonic Extraction Process of Four Active Components from Salviae Miltiorrhizae Radix et Rhizoma by Uniform Design

更新时间：2024-01-04

- Optimization of Rapid Ultrasonic Extraction Process of Four Active Components from Salviae Miltiorrhizae Radix et Rhizoma by Uniform Design
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 10, Pages: 8-12(2014)
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- DOI：10.13422/j.cnki.syfjx.2014100008
  CLC： R284.2
- Published：2014
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GAO Shu-rui, ZHAO Zhi-gang, HOU Jun-ling, et al. Optimization of Rapid Ultrasonic Extraction Process of Four Active Components from Salviae Miltiorrhizae Radix et Rhizoma by Uniform Design[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 8-12.
DOI：

GAO Shu-rui, ZHAO Zhi-gang, HOU Jun-ling, et al. Optimization of Rapid Ultrasonic Extraction Process of Four Active Components from Salviae Miltiorrhizae Radix et Rhizoma by Uniform Design[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 8-12. DOI： 10.13422/j.cnki.syfjx.2014100008.

摘要

目的：优选丹参中迷迭香酸、隐丹参酮、丹酚酸B及丹参酮ⅡA共4种活性成分的超声提取工艺。方法：以迷迭香酸、隐丹参酮、丹酚酸B及丹参酮ⅡA提取量为指标，采用均匀设计考察乙醇体积分数、液料比、提取时间对丹参提取效率的影响，通过逐步非线性回归优选提取工艺条件。利用HPLC测定指标成分含量，流动相甲醇（A）-0.01%磷酸水（B）梯度洗脱（0~3 min，30%A；3~5 min，30%~40%A；5~13 min，40%A；13~20 min，40%~58%A；20~22 min，58%~75%A；22~24 min，75%A；24~50 min，75%~85%A），检测波长286 nm（迷迭香酸和丹酚酸B）和268 nm（隐丹参酮和丹参酮ⅡA）。结果：最佳超声提取工艺条件为乙醇体积分数75%，液料比200 mL·g-1，提取时间10 min；迷迭香酸、丹酚酸B、隐丹参酮及丹参酮ⅡA提取量分别为0.33，11.19，0.63，0.59 g·g-1，与2010年版《中国药典》中加热回流法相比，活性成分提取量无显著差异。结论：该超声提取工艺具有提取时间短、低毒、无污染等优点。

Abstract

Objective: To optimize ultrasonic extraction technology of rosmarinic acid

salvianolic acid B

cryptotanshinone and tanshinone ⅡA from Salviae Miltiorrhizae Radix et Rhizoma. Method: With extraction amounts of four active components as indexes

uniform design was adopted to optimize extraction technology by taking ethanol concentration

solid-liquid ratio

extracting time as factors.HPLC was employed to determine contents of four active components with mobile phase of methanol-0.01% phosphoric acid solution(0-3 min

30%A;3-5 min

30%-40%A;5-13 min

40%A;13-20 min

40%-58%A;20-22 min

58%-75%A;22-24 min

75%A;24-50 min

75%-85%A) for gradient elution and detection wavelengths at 286 nm(rosmarinic acid and salvianolic acid B) and 268 nm(cryptotanshinone and tanshinone ⅡA). Result: Optimum extraction conditions were as follows:ethanol concentration of 75%

liquid-solid ratio of 200 mL·g-1

extraction time 10 min;Under these conditions

extraction amounts of rosmarinic acid

salvianolic acid B

cryptotanshinone and tanshinone ⅡA were 0.33

11.19

0.63 and 0.59 g·g-1

respectively.Compared with heating reflux extraction in 2010 edition of "Chinese Pharmacopoeia"

Contents of four bioactive components had no significant differences. Conclusion: This optimum extraction process had advantages of low mammalian toxicity

solvent saving

fast extracting speed and so on.

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