Objective: To optimize extraction technology of compound Smilacis Glabrae Rhizoma granules. Method: With overall desirability(OD) of extraction rate of astilbin and total flavonoids

dry extract yield as dependent variable

Box-Behnken response surface methodology was employed to optimize extraction technology by taking extraction times

extraction time and solid-liquid ratio as independent variables

each level of independent variables was fitted by multiple linear regression and binomial formula fitting.HPLC was adopted to determine the content of astilbin with mobile phase of acetonitrile-0.1% phosphoric acid(20:80) and detection wavelength at 290 nm. Result: Optimum extraction technology was as following:extracted 3 times with 9.8-fold the amount of water for 1.4 h each time;under these conditions

extraction rates of astilbin and total flavonoids were 1.991 and 54.436 mg·g-1

dry extract yield was 8.558%

relative deviation between the observed and predicted values was 1.54%. Conclusion: Box-Behnken response surface methodology was suitable for optimizing extraction technology of compound Smilacis Glabrae Rhizoma granules.