HU Yu-fei, LIU Xuan-lin, HE Cheng-hui, et al. Optimization on Preparation Process of Total Flavonoids from -phospholipids Complex by Central Composite Design and Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 36-39.
DOI:
HU Yu-fei, LIU Xuan-lin, HE Cheng-hui, et al. Optimization on Preparation Process of Total Flavonoids from -phospholipids Complex by Central Composite Design and Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 36-39. DOI: 10.13422/j.cnki.syfjx.2014100036.
Optimization on Preparation Process of Total Flavonoids from -phospholipids Complex by Central Composite Design and Response Surface Methodology
Objective: To optimize preparation process of total flavonoids from Dracocephalum moldevica-phospholipids complex. Method: Solvent evaporation method was adopted to prepare total flavonoids from D. moldevica-phospholipids complex.Based on single factor tests
with recombination rate as dependent variable
central composite design was adopted to optimize preparation technology by taking reaction time
ratio of total flavonoids to phospholipids and the concentration of total flavonoids as independent variables
fitting of various mathematical equations were performed using a statistical software of Design-Expert 8.0.6.Preparation parameters were optimized through response surface plotted by optimum fitting equations
optimized procedure was validated through experimental preparation of total flavonoids-phospholipids complex. Result: Optimum preparation technology was as following:reaction temperature 40 ℃
with methanol-dioxane(7:3) as a reaction solvent
ratio of phospholipids to total flavonoids of 2:1
total flavonoids concentration of 15 g·L-1 and reaction time 2 h;Under these conditions
recombination rate was up to (96.42±1.58)%
which had deviation of <2.0% between the observed value of recombination rate. Conclusion: Optimized preparation technology was feasible
reliable and suitable for industrial production of total flavonoids from D. moldevica-phospholipids complex.