Determination of Aspidin BB in  by UPLC

TANG Wen-wei; SHEN Zhi-bin; YIN Yong-qin; WU Jie-ying; ZHANG Hui; HUANG Yong-chang

doi:10.13422/j.cnki.syfjx.2014100051

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Determination of Aspidin BB in by UPLC

更新时间：2024-01-04

- Determination of Aspidin BB in by UPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 10, Pages: 51-53(2014)
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- DOI：10.13422/j.cnki.syfjx.2014100051
  CLC： R284.1
- Published：2014
- 稿件说明：
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TANG Wen-wei, SHEN Zhi-bin, YIN Yong-qin, et al. Determination of Aspidin BB in by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 51-53.
DOI：

TANG Wen-wei, SHEN Zhi-bin, YIN Yong-qin, et al. Determination of Aspidin BB in by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 51-53. DOI： 10.13422/j.cnki.syfjx.2014100051.

摘要

目的：建立超高效液相色谱法测定香鳞毛蕨药材中绵马素 BB的含量。方法：采用XBridgeTM BEH C18（4.6 mm×100 mm，2.5 μm）色谱柱，以乙腈-0.1%三氟乙酸水溶液（90∶10）为流动相，流速0.80 mL·min-1，检测波长290 nm，柱温40 ℃，进行测定。结果：绵马素BB在0.050 28~0.301 68 μg线性关系良好（r=0.999 8），平均回收率为98.87%（RSD 0.44%）。结论：该方法快速、准确、重复性好，可用于香鳞毛蕨的含量测定和质量控制。

Abstract

Objective: To establish an UPLC method for the content determination of aspidin BB in Dryopteris fragrans. Method: Determination was performed on XBridgeTM BEH C18 column (4.6 mm×100 mm

2.5 μm) with mobile phase consisted of acetonitrile-0.1% trifluoroacetic acid with detection wavelength at 290 nm. The flow rate was set to 0.8 mL·min-1

while the column temperature was set at 40 ℃. Result: The linear range of aspidin BB was 0.050 28-0.301 68 μg(r=0.999 8)with an average recovery of 98.87%(RSD 0.44%

n=6). Conclusion: The method is fast

accurate and reproducible

which can be used to control the quality of D. fragrans.

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Related Institution

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

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