Objective: To establish the methods for extraction

isolation and determ ination of ellagic acid from Turpinia arguta

and to compare its contents from different areas. Method: The ellagic acid reference substance was isolated by solvent extraction

macroporous adsorption resin

reverse phase silica gel column chromatog ramphy and recrystallization techniques. Then determined it by the method of HPLC from T. arguta

the chromatographic column was UltimateXB-C18 column(4.6 mm×250 mm

5 μm)

the mobile phase was acetonitrile-0.1% phosphoric acid

the flow rate was at 1.0 mL·min-1

the detection wavelength was 254 nm and the column temperature was 30 ℃. Result: The purity of ellagic acid obtained was higher than 98.5%

and the average recovery was 101.25%.The good linearity was achieved in the range of 0.020 0-0.400 8 μg. Ellagic acid content of T. arguta from 10 different areas was between 0.37% and 1.56%. Conclusion: Ellagic acid obtained from the method was with high purity

and it can be adopted as the reference substance. This method was simple

rapid and accuracy

it can be used to determine the contents of ellagic acid from T. arguta .The contents of ellagic acid were different in T. arguta from different areas. The result provided a reference for development of T. arguta resource.