Determination of Genistin, Genistein,Protocatechuic Acid and Gallic Acid in MZSYJ by HPLC

ZHUO Shen; CHEN Jun; QIN Hai-guang

doi:10.13422/j.cnki.syfjx.2014100072

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Determination of Genistin, Genistein,Protocatechuic Acid and Gallic Acid in MZSYJ by HPLC

更新时间：2024-01-04

- Determination of Genistin, Genistein,Protocatechuic Acid and Gallic Acid in MZSYJ by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 10, Pages: 72-76(2014)
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- DOI：10.13422/j.cnki.syfjx.2014100072
  CLC： R284.1
- Published：2014
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ZHUO Shen, CHEN Jun, QIN Hai-guang. Determination of Genistin, Genistein,Protocatechuic Acid and Gallic Acid in MZSYJ by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 72-76.
DOI：

ZHUO Shen, CHEN Jun, QIN Hai-guang. Determination of Genistin, Genistein,Protocatechuic Acid and Gallic Acid in MZSYJ by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 72-76. DOI： 10.13422/j.cnki.syfjx.2014100072.

摘要

目的：采用高效液相梯度洗脱法测定马鬃蛇药酒（MZSYJ）中染料木苷、染料木素、原儿茶酸和没食子酸的含量。方法：Hypersil C18色谱柱（4.6 mm×200 mm，5 μm）；流速1.0 mL·min-1。染料木苷和染料木素：流动相A为乙腈-甲醇（2∶1），流动相B为0.2%甲酸水溶液，检测波长261 nm。原儿茶酸和没食子酸：流动相A为甲醇，流动相B为0.3%磷酸溶液，检测波长268 nm。结果：染料木苷和染料木素分别在0.010 4~0.208 0 μg（r=0.999 3），0.004 8~0.096 0 μg（r=0.999 6）进样量与峰面积呈良好的线性关系，平均加样回收率分别为97.18%，96.94%，RSD分别为1.52%，1.40%；原儿茶酸和没食子酸分别在0.038 2~0.764 0 μg（r=0.999 1），0.011 6~0.232 0 μg（r=0.999 4）进样量与峰面积呈良好的线性关系，平均加样回收率分别为95.97%，98.39%，RSD分别为1.10%，1.45%。结论：该方法测定结果准确、灵敏、重复性好。

Abstract

Objective: To develop an HPLC method for determination of the content of genistin

genistein

protocatechuic acid and gallic acid in Mazongshe Yaojiu(MZSYJ). Method: An Hypersil C18 column was used as the chromatographic column

the flow rate was 1.0 mL·min-1. Genistin and genistein:the mobile phase A consisted of acetonitrile-methanol(2:1)

the mobile phase B consisted of 0.2% formic acid solution

the UV detection wavelength was at 261 nm. Protocatechuic acid and gallic acid:the mobile phase A consisted of methanol

the mobile phase B consisted of 0.3% phosphoric acid solution. The UV detection wavelength was at 268 nm. Result: There were a good linear relationship between the concentration of genistin

genistein and peak area value when the concentrations of genistin and genistein were within the range of 0.010 4-0.208 0 μg(r=0.999 3)

0.004 8-0.096 0 μg(r=0.999 6). The average recovery were 97.18%(RSD 1.52%) and 96.94%(RSD 1.40%). There were a good linear relationship between the concentration of protocatechuic acid

gallic acid and peak area value when the concentrations of protocatechuic acid and gallic acid were within the range of 0.038 2-0.764 0 μg(r=0.999 1)

0.011 6-0.232 0 μg(r=0.999 4).The average recovery were 95.97%(RSD 1.10%) and 98.39%(RSD 1.45%). Conclusion: The method was accurate

sensitive

reproducible and may be used in the of genistin

genistein

protocatechuic acid and gallic acid in MZSYJ.

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