DU Zhong-ying, QI Jin, YU Bo-yang. Simultaneous Determination of Seven Components in Zengye Decoction by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 76-79.
DOI:
DU Zhong-ying, QI Jin, YU Bo-yang. Simultaneous Determination of Seven Components in Zengye Decoction by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 76-79. DOI: 10.13422/j.cnki.syfjx.2014100076.
Simultaneous Determination of Seven Components in Zengye Decoction by HPLC
Objective: To establish a HPLC method for simultaneous determination of 5-HMF
harpagoside
acteoside
angoroside C
cinnamic acid
methylophiopogonanone A and methylophiopogonanone B in Zengye Decoction. Method: A Diamonsil C18 (4.6 mm×250 mm
5 μm) was used;The mobile phase was acetonitrile-0.02% formic acid in gradient elution at flow rate1.0 mL·min-1 detected at 296 nm;The column temperature was maintained at 30 ℃. Result: The linear range (mg·L) of 5-HMF
acteoside
angoroside C
harpagoside
cinnamic acid
methylophiopogonanone A and methylophiopogonanone B was 4.12-165.00
3.98-159.20
6.97-278.80
7.25-290.00
4.29-171.60
0.07-2.80
0.12-5.00. The average recoveries were 97.42%
96.97%
97.58%
96.94%
99.16%
99.46% and 100.33% respectively. Conclusion: The developed method is convenient
reliable and accurate for the quality control of Zengye Decoction.
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