Screening Test for Effective Fraction of Antithrombotic Action from Typhae Pollen

HE Guo-lin; ZHOU Wei-jun; WANG Ling-li; LI Yuan-bin; QIANG Jiao; CHEN Yan-yan

doi:10.13422/j.cnki.syfjx.2014100138

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Screening Test for Effective Fraction of Antithrombotic Action from Typhae Pollen

更新时间：2024-01-04

- Screening Test for Effective Fraction of Antithrombotic Action from Typhae Pollen
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 10, Pages: 138-141(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014100138
  CLC： R285.5
- Published：2014
- 稿件说明：
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HE Guo-lin, ZHOU Wei-jun, WANG Ling-li, et al. Screening Test for Effective Fraction of Antithrombotic Action from Typhae Pollen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 138-141.
DOI：

HE Guo-lin, ZHOU Wei-jun, WANG Ling-li, et al. Screening Test for Effective Fraction of Antithrombotic Action from Typhae Pollen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 138-141. DOI： 10.13422/j.cnki.syfjx.2014100138.

摘要

目的：确定蒲黄抗血栓作用的主要活性部位。方法：对蒲黄不同提取部位（石油醚、乙酸乙酯、正丁醇、水）对体外血小板聚集作用进行筛选，进一步研究蒲黄活性部位对胶原蛋白-肾上腺素和角叉菜胶诱发小鼠体内血栓形成的影响，蒲黄有效部位对大鼠体内血栓模型及血管壁的作用。结果：蒲黄乙酸乙酯部位对花生四烯酸、二磷酸腺苷、胶原、凝血酶诱发家兔体外血小板聚集的抑制率分别为40.08%，33.09%，37.91%，34.71%，水部位则为29.29%，28.65%，28.33%，32.29%；而蒲黄乙酸乙酯部位对胶原蛋白-肾上腺素诱发小鼠死亡和偏瘫的保护率为50%，对角叉菜胶诱发小鼠黑尾发生率为50%；同时乙酸乙酯部位可明显抑制大鼠体内血栓的形成，并对血管内皮有明显的保护作用。结论：初步认为乙酸乙酯部位为蒲黄抗血栓作用的主要活性部位。

Abstract

Objective: To ascertain the main active site of Typhae Pollen in effect of antithrombosis. Method: Screening for effect of platelet aggregation in vitro from the different extracts of Typhae Pollen(petroleum ether

ethyl acetate

butanol

water)was carried out

further study the effects of the active sites of Typhae Pollen on the collagen-adrenaline and carrageenan induced thrombosis of mice. Effect of effective parts of Typhae Pollen for the rats of thrombosis and vascular wall in vivo. Result: The ethyl acetate parts of Typhae Pollen on arachidoric acid(AA)

adenosine diphosphate(ADP)

collagen

thrombin induced rabbit platelet aggregation inhibition rates were 40.08%

33.09%

37.91%

34.71%

29.29%

compared with the water parts were 28.65%

28.33%

32.29%;while the protection rate of collagen-adrenaline induced death and paralysis in mice by the ethyl acetate parts of Typhae Pollen was 50%

carrageenan-induced mice black tail incidence was 50%;the site of ethyl acetate have significantly inhibitive effect on the thrombosis models in vivo

and significantly protective effect on vascular endothelial. Conclusion: The site of ethyl acetate is the main active site of the Typhae Pollen.

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Related Institution

State Key Laboratory of Functions and Applications of Medicinal Plants, Provincial Key Laboratory of Pharmaceutics in Guizhou Province, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University

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