Mechanism of Pachouli Alcohol Inhibiting Growth of Human Androgen-independent Prostate Cancer Cell Line DU145

CAI Jian; PENG Cheng; ZHU Xiao-yan; ZHANG Cheng; WAN Feng; ZENG Wen-tong; ZHANG Shu-wu

doi:10.13422/j.cnki.syfjx.2014100165

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Mechanism of Pachouli Alcohol Inhibiting Growth of Human Androgen-independent Prostate Cancer Cell Line DU145

更新时间：2024-01-04

- Mechanism of Pachouli Alcohol Inhibiting Growth of Human Androgen-independent Prostate Cancer Cell Line DU145
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 10, Pages: 165-169(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014100165
  CLC： R285.5
- Published：2014
- 稿件说明：
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CAI Jian, PENG Cheng, ZHU Xiao-yan, et al. Mechanism of Pachouli Alcohol Inhibiting Growth of Human Androgen-independent Prostate Cancer Cell Line DU145[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 165-169.
DOI：

CAI Jian, PENG Cheng, ZHU Xiao-yan, et al. Mechanism of Pachouli Alcohol Inhibiting Growth of Human Androgen-independent Prostate Cancer Cell Line DU145[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 165-169. DOI： 10.13422/j.cnki.syfjx.2014100165.

摘要

目的：观察广藿香醇（pachouli alcohol，PA）对人雄激素非依赖性前列腺癌细胞DU145的凋亡诱导效应及增殖抑制作用，探讨其发生机制。方法：广藿香醇10，20，40，80，160 mg·L-1作用在密度为6×107/L DU145细胞24，48，72 h 后，采用四甲基偶氮唑盐法（MTT）检测广藿香醇对细胞的生长抑制作用；透射电镜观察药物诱导细胞凋亡形态变化；Annexin V-FITC，PI双标记法流式细胞仪检测药物诱发细胞凋亡的改变；Western blot检测半胱氨酸天冬氨酸蛋白酶3（Caspase-3）、B细胞淋巴瘤/白血病-2蛋白（Bcl-2）、Bcl-2关联X蛋白（Bax）、凋亡抑制蛋白Livin的表达。结果：MTT法检测提示广藿香醇处理组细胞增殖抑制作用明显，10，20，40，80，160 mg·L-1广藿香醇对DU145的24，48，72 h抑制率分别为5.73%，16.67%，22.61%；13.42%，25.03%，39.68%；25.92%，31.46%，52.76%；39.61%，54.68%，73.52%；56.42%，77.35%，91.58%，呈剂量-时间依赖性（P<0.05）。透射电镜显示药物作用后细胞出现凋亡形态改变。流式细胞仪检测提示广藿香醇作用使细胞凋亡率明显升高，与对照组比较有统计学意义（P<0.05）。Western blot检测提示广藿香醇可增强细胞Caspase-3，Bax的表达，下调Livin，Bcl-2蛋白的表达。结论：广藿香醇能抑制DU145细胞增殖，其作用机制可能与诱导细胞凋亡效应有关。

Abstract

Objective: To observe the apoptosis inducing effect and inhibitory effect of pachouli alcohol on the proliferation of human androgen independent prostate cancer cell line DU145

and its mechanisms. Method: After prostate cancer DU145 cells were treated with pachouli alcohol for 24

72 h

the apoptosis morphology was observed by transmission electron microscope;cell viability was estimated using MTT assay;cell apoptosis rate was measured by flow cytometry. Western blot was used to test Caspase-3

Bcl-2

Bax and Livin protein expression. Result: Apoptosis of DU145 cells was detected by transmission electron microscopy after using pachouli alcohol. In vitro

pachouli alcohol significantly inhibited the proliferation of DU145 cells in dose and time-dependent manners which was detected by MTT method. The apoptosis rate was increased significantly which compare with the control group. Pachouli alcohol could enhance Caspase-3

Bax protein expression

and reduce Livin

Bcl-2 protein expression in DU145 cells. Conclusion: Pachouli alcohol can inhibit the proliferation of DU145 in vitro

which may be correlated with inducing the cell apoptosis.

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