Studies on Antitumor Effects of  and its Material Basis

WEI Jin-bin; NONG Cai-li; SU Zhi-heng; CHEN Yong-xin; LV Shu-juan; PAN Yu-zheng

doi:10.13422/j.cnki.syfjx.2014100169

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Studies on Antitumor Effects of and its Material Basis

更新时间：2024-01-04

- Studies on Antitumor Effects of and its Material Basis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 10, Pages: 169-174(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014100169
  CLC： R285.5
- Published：2014
- 稿件说明：
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WEI Jin-bin, NONG Cai-li, SU Zhi-heng, et al. Studies on Antitumor Effects of and its Material Basis[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 169-174.
DOI：

WEI Jin-bin, NONG Cai-li, SU Zhi-heng, et al. Studies on Antitumor Effects of and its Material Basis[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 169-174. DOI： 10.13422/j.cnki.syfjx.2014100169.

摘要

目的：观察金花茶3种不同萃取部位对5种不同人肿瘤细胞株体外增殖的作用，寻找其活性部位及物质基础。方法：采用噻唑蓝染色法（MTT法），检测金花茶不同萃取部位体外对人胃腺癌细胞株（SGC-7901）、人大细胞肺癌细胞株（H460）、人肝癌细胞株（SMMC-7721和BEL-7404）、人高分化鼻咽癌细胞株（CNE-1）作用48 h后的增殖抑制率，并计算相应的半数生长抑制浓度（IC50），其中，细胞密度分别为：SGC-7901（7×104/mL），H460（4×104/mL），SMMC-7721（6×104/mL），BEL-7404（6×104/mL），CNE-1（4×104/mL），不同提取部位的浓度分别为：正丁醇层（50，100，150，200 mg·L-1）、乙酸乙酯层（50，100，200，300 mg·L-1）、水层（25，50，75，100，150 mg·L-1）；采用液质联用方法初步分析水层萃取部分的可能活性成分。结果：金花茶正丁醇、乙酸乙酯、水层部位对5种肿瘤均有抑制作用（P<0.05或P<0.01），且呈现一定的浓度依赖性，各部分对5种肿瘤细胞的抑制作用强弱为：水层>正丁醇层>乙酸乙酯层，水层部位对上述5种细胞株的IC50分别为：81.72，73.47，95.98，73.41，61.25 mg·L-1；水层萃取部分检测到的物质有24个，初步鉴定了其中的11个成分[分别为:（1R，3R，4R，5R）-1，3，4，5-tetrahydroxycyclohexanecarboxylic acid;daucic acid;二羟基-6-甲氧基-四氢-2H-吡喃-3-氧基-四氢-2H-吡喃-3，4，5-三醇;coumaroylquinic acid;1，3，4-tri-O-acetyl-alpha-D-fructofuranosyl，3，6-tri-O-acetyl-alpha-D-glucopyranoside;2S，3aR， 4’S，5’S，6R，6’R，7R，7aS）-6，6’-bis[（acetyloxy）methyl]tetrahydro-4H，4’H-spiro[1，3-dioxolo[4，5-c]pyran-2，3’-pyran]-3a，4’，5’，7（6H）-tetrayl tetraacetate；（2S，3R，4R，5R，6S）-2-（2S，3R，4S，5S，6R）-4，5-二羟基-2-（5-羟基-7-甲氧基-2-（4-甲氧苯基）-4-羰基-4H-色烯-6-基）-6-（羟甲基）-四氢-2H-吡喃-3-氧基-4，5-二羟基-6-甲基-四氢-2H-吡喃-3-基乙酸酯；vitexin-7-glucoside；luteolin-7-diglucoside；7-O-β-D-glucopyranosyl-kaempferol-3-O-α-L-rhamnopyranosyl-（1→2）-β-D-glucopyranoside；ilexside Ⅱ]，同时表征了另外13个未鉴定成分的质谱信息。结论：金花茶不同萃取部位体外实验有抗肿瘤活性，水层部分可能是金花茶抗肿瘤作用的主要活性部位；需要进一步对水层部分的这些可能活性物质进行分离纯化鉴定以及定量分析。

Abstract

Objective: To observe the inhibitory effects of different extractive fractions from Camellia chrysantha on five different kinds of malignant tumor cell lines

and evaluate the antitumor effects in vitro and its material basis of the effects. Method: MTT assay was adopted to detect the inhabitation of different extractive fractions from C. chrysantha with five different kinds of malignant tumor cell lines in vitro and IC50 values were calculated

respectively. The UPLC-LTQ-Orbitrap MS/MS was used to analyse its possible components. Result: The different extractive fractions from C. chrysantha had antitumor effects on SGC-7901

H460

SMMC-7721

BEL-7404

CNE-1

they inhibited cell proliferation in a dose dependent manner. The sequence of their inhabitation intensity was:water-soluble part>n-butyl alcohol part>ethyl acetate part

and the IC50 of water-soluble part on the five different kinds cell lines was 81.72

73.47

95.98

73.41

61.25 mg·L-1

respectively;24 components were investigated

11 of them were tentatively identified

and 13 of them were characterized by their mass spectra information. Conclusion: Different extractive fractions from C. chrysantha can inhibit the proliferation of SMMC-7721

BEL-7404

CNE-1

SGC-7901 and H460 in vitro

the water-soluble part is considered as antitumor active part of C. chrysantha;but which component exactly contribute to the antitumor effect remains to be further confirmed.

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