Objective: To establish the scald model on skins of two sides of buttock in mice by punctate-like scald device that we invested. To compare the therapeutic effects of different yolk oils which prepared by fried fresh yolk based on the model we established. Method: Scald model was established. Healthy Kunming mice

half male and half female

were used in the model. The mice were divided into two groups

high weight group(33.44±3.97) g and low weight group (27.42±1.66) g. The mice were fixed by adhesive bands. A 1.5 cm-diameter circle scalded area was made on each side of buttock of the mice by punctate-like scald device. The time that the scalded area of the mice began to scab at first and the skin samples of scalded area of the mice were collected and analyzed. The effect on the model we established by the time that the scald device covered the skin and the weight of the mice were explored. The efficacy of different drugs were compared. After the mold was built

the mice were randomly divided into 14 groups

namely model control group

positive control group that treated with silver sulfadiazine cream

egg yolk oil treatment group

duck yolk oil treatment group concluding

egg yolk oil and duck yolk treatment group

egg oil and silver sulfadiazine cream treatment group

yolk oil and silver sulfadiazine cream treatment group. Each group all divided into male group and female group. Healing times of scald wound of each group were recorded. The effect of the drugs were compared according to the data we obtained. Result: There are successful models in the groups

concluding the group which contacting time was 5 s with two weights of mice

contacting time was 10 s with high weight of mice. The mice were all died in the groups of contacting time was 10 s with low weight of mice and contacting time was 25 s of two weights of mice. The time that the scalded area of the mice began to scab at first and degree of injure were affected by the weight of mice. In 5 s group

the time was significant different between the high weight and low weight(P<0.01). In the male mice group

healing time was reduced significantly in positive control group than in model control group(P<0.01). There was no significant difference between positive control group and egg-duck yolk treatment group(P>0.05). In this experiment

the healing effect of duck yolk oil was not as good as egg yolk oil in this test(P<0.01)

female mice showed a stronger self-healing ability(P<0.01) than male mice. Healing effect of duck yolk oil has significant sexual difference(P<0.01)

while the egg yolk oil has no such cases(P>0.05). Conclusion: The skin scald model has character of good replicability because the punctate-like scald device we invested in this experiment is easy to control the scald temperature. The duck yolk oil has significant sexual difference(P<0.01) in healing wound and its effect is less than the egg yolk oil.