Effect of Polysaccharide Isolated from Agaricus Blazei Murill on mRNA Expression Levels of Dectin-1, Syk and CARD9 from Dendritic Cells

DONG Hai-ying; GAO Zhi-ying; RONG Hua; JIN Ming; WANG Jun-ping; NIU Ying-cai; LIU Ji-cheng

doi:10.13422/j.cnki.syfjx.2014100180

您当前的位置：

首页 >

文章列表页 >

Effect of Polysaccharide Isolated from Agaricus Blazei Murill on mRNA Expression Levels of Dectin-1, Syk and CARD9 from Dendritic Cells

更新时间：2024-01-04

- Effect of Polysaccharide Isolated from Agaricus Blazei Murill on mRNA Expression Levels of Dectin-1, Syk and CARD9 from Dendritic Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 10, Pages: 180-183(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014100180
  CLC： R730.5
- Published：2014
- 稿件说明：
移动端阅览
DONG Hai-ying, GAO Zhi-ying, RONG Hua, et al. Effect of Polysaccharide Isolated from Agaricus Blazei Murill on mRNA Expression Levels of Dectin-1, Syk and CARD9 from Dendritic Cells[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 180-183.
DOI：

DONG Hai-ying, GAO Zhi-ying, RONG Hua, et al. Effect of Polysaccharide Isolated from Agaricus Blazei Murill on mRNA Expression Levels of Dectin-1, Syk and CARD9 from Dendritic Cells[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(10): 180-183. DOI： 10.13422/j.cnki.syfjx.2014100180.

摘要

目的：探讨低相对分子质量姬松茸多糖（low molecular weight polysaccharide isolated from Agaricus blazei Murill，LMPAB）对树突状细胞C型植物血凝素1（Dectin-1），脾酪氨酸激酶（Sky）和胱天蛋白募集域蛋白9（CARD9）基因表达和T淋巴细胞增殖的影响。方法：体外培养 ICR小鼠骨髓来源的树突状细胞；通过流式细胞术检测树突状细胞表面CD11c和CD86分子的表达；采用WST方法检测LMPAB增强树突状细胞对T淋巴细胞的增殖作用；应用实时定量PCR方法检测Dectin-1，Sky和CARD9 mRNA的表达水平。结果：树突状细胞表面CD11c表达98.6%，CD86表达96.97%；与正常组（4.84±0.21）比较，LMPAB +OVA组（6.59±0.19）可增强树突状细胞对T淋巴细胞的增殖作用（P<0.05）；与正常组Dectin-1（6.13±0.27），Sky（0.36±0.11）和CARD9（1.85±0.20）比较，LMPAB +OVA组Dectin-1（8.25±0.19），Sky（1.63±0.16）和CARD9（4.40±0.19）明显升高，统计学具有显著意义（P<0.05）。结论：所培养的髓系细胞为树突状细胞；LMPAB可通过调控树突状细胞Dectin-1，Sky和CARD9 mRNA的表达增强树突状细胞的免疫效能。

Abstract

Objective: To investigate the effect of low molecular weight polysaccharide isolated from Agaricus blazei Murill (LMPAB) on Dectin-1

Syk and CARD9 mRNA expression of dendritic cells. Method: Dendritic cells derived from bone marrow of ICR mice were cultured in vitro; the expression of CD11c and CD86 molecule on the surface of dendritic cells was detected by flow cytometry; the effect of LMPAB on the proliferation of T lymphocytes stimulated by dendritic cells was detected by WST. Real-time quantitative PCR detect Dectin-1 mRNA

Sky mRNA and CARD9 mRNA expression levels. Result: CD11c and CD86 protein expression were expressed by 98.6% and 96.97% respectively; compared with the control group (4.84±0.21)

LMPAB+OVA group (6.59±0.19) enhances the effect of dendritic cells on stimulating T lymphocytes' proliferation (P<0.05). Compared with the mRNA expression of Dectin-1 (6.13±0.27)

Sky (0.36±0.11) and CARD9(1.85±0.20) in the control group

Dectin-1 (8.25±0.19)

Sky (1.63±0.16) and CARD9 (4.40±0.19) mRNA expression in LMPAB+OVA group was significantly increased with statistically significant (P<0.05). Conclusion: The cells derived from bone marrow were dendritic cells; immunizing potency could be enhanced by LMPAB regulating and controlling Dectin-1

Sky

CARD9 mRNA expression levels in Dendritic cells.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Icariin on Myocardial Remodeling in Rats Based on Vitamin D Regulation of Dendritic Cell Phenotype

Related Author

LI Qian

CHEN Yujia

ZHOU Yan

LI Wen

GUAN Liancheng

WANG Huanzhen

CHEN Yunzhi

Related Institution

Guizhou University of Traditional Chinese Medicine（TCM）

The Second Affiliated Hospital of Guizhou University of TCM

Dejiang Nation Hospital of TCM

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰