Objective:To investigate pharmacological action of lactone part and fat part in alcohol extracts of roasted Aucklandiae Radix

then optimize purification technology of the alcohol extracts. Method:Taking ink propulsion rate in the small intestine and diarrhea index as indexes

effects of lactone part and fat part in alcohol extracts of roasted Aucklandiae Radix after cold storage on diarrhea induced by Sennae Folium in healthy mice were investigated.With composite score of yield of dry extract

transfer rates of costunolide and dehydrocostus lactone as index

orthogonal test was adopted to optimize purification technology by taking ethanol concentration and consumption

refrigeration temperature and time as factors. Result:Propulsive motility in the small intestine and diarrhea index could be reduced by lactone part

while fat part had opposite effects with slippery bowel purgative effect.The best purification technology was as follows:refrigerated 5 h with 5 times the amount of 80% ethanol at -10 ℃;yield of dry extract

transfer rates of costunolide and dehydrocostus lactone were 5.1%

94.9%

93.8%

respectively. Conclusion:Fat part in alcohol extracts of roasted Aucklandiae Radix should be removed.This optimized refrigeration process was simple and feasible

based on in favor of preparation

it could remove fat part with slippery bowel purgative effect

and had a certain reference value in actual production.