ZHU Jin-kun, ZHANG Quan-wei, ZHANG Yi-dan, et al. Optimization of Enrichment and Purification Process for Total Flavonoids from Gossampini Flos by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 19-22.
DOI:
ZHU Jin-kun, ZHANG Quan-wei, ZHANG Yi-dan, et al. Optimization of Enrichment and Purification Process for Total Flavonoids from Gossampini Flos by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 19-22. DOI: 10.13422/j.cnki.syfjx.2014110019.
Optimization of Enrichment and Purification Process for Total Flavonoids from Gossampini Flos by Macroporous Resin
Objective:To optimize enrichment and purification technology of total flavonoids from Gossampini Flos with macroporous resin. Method:The content of total flavonoids was determined by UV
taking adsorption rate and desorption rate as index
resin type was screened by static adsorption-elution test
single factor tests were adopted to investigate effects of the concentration of sample solution
pH
adsorption rate
desorption solvent
water amounts
the amount of sample solution and other factors on purification process. Result:It was proved that DM-130 resin could effectively enrich total flavonoids from Gossampini Flos
optimum purification process was as following:the concentration of sample solution 4.89 g·L-1
pH of 4.0
flow rate for sampling of 3 BV·h-1
removed impurities by 5 BV of water and then eluted total flavonoids with 3 BV of 50% ethanol at flow rate of 2.0 BV·h-1;purity of tota1 flavonoids increased from 13.33% in sample solution to 77.68% in eluant. Conclusion:DM-130 resin could be used for purification and enrichment of tota1 flavonoids from Gossampini Flos
which had high industrial production value with characteristics of easy operation