LI Wan-wan, LIANG Jing-kang, HU Qiao-hong. Optimization of Extraction Technology of Sanzi Prescription by Orthogonal Test Combined with Asthma Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 26-30.
DOI:
LI Wan-wan, LIANG Jing-kang, HU Qiao-hong. Optimization of Extraction Technology of Sanzi Prescription by Orthogonal Test Combined with Asthma Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 26-30. DOI: 10.13422/j.cnki.syfjx.2014110026.
Optimization of Extraction Technology of Sanzi Prescription by Orthogonal Test Combined with Asthma Test
Objective:To optimize extraction process of Sanzi prescription. Method:Asthma test induced by histamine on guinea pigs was used to compare decoction and ethanol reflux extraction.With composite score of contents of sinapine thiocyanate and rosmarinic acid
yield of extract as index
orthogonal test was designed to optimize extraction technology by taking ethanol concentration
solvent consumption
extracting time and times as factors
optimal process was verified with asthma test induced by histamine on guinea pigs. Result:Ethanol reflux extraction was adopted
optimal extraction conditions were as follows:extracted thrice with ten times the amount of 70% ethanol for 1 h each time;under these conditions
extracting amounts of sinapine thiocyanate and rosmarinic acid were 7.26
5.63 mg·g-1
respectively. Conclusion:This extraction process was reasonable and reproducible
which was suitable for industrial production of Sanzi prescription.
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SU Ying-hong
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LIU Dewen
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Related Institution
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences
Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine
Graduate School,Heilongjiang University of Chinese Medicine
Medical School, Lijiang Culture and Tourism College