Determination of Chlorogenic Acid and Caffeic Acid in  from Xinjiang by HPLC

GONG Jian; LU Shuai; SUO Fei-ya; WANG Ao-li; YE Xing

doi:10.13422/j.cnki.syfjx.2014110050

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Determination of Chlorogenic Acid and Caffeic Acid in from Xinjiang by HPLC

更新时间：2024-01-04

- Determination of Chlorogenic Acid and Caffeic Acid in from Xinjiang by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 11, Pages: 50-52(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014110050
  CLC： R284.1
- Published：2014
- 稿件说明：
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GONG Jian, LU Shuai, SUO Fei-ya, et al. Determination of Chlorogenic Acid and Caffeic Acid in from Xinjiang by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 50-52.
DOI：

GONG Jian, LU Shuai, SUO Fei-ya, et al. Determination of Chlorogenic Acid and Caffeic Acid in from Xinjiang by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 50-52. DOI： 10.13422/j.cnki.syfjx.2014110050.

摘要

目的：建立HPLC测定新疆产短星火绒草中绿原酸和咖啡酸含量的方法，并用于短星火绒草中绿原酸和咖啡酸含量的测定。方法：采用DiamonsilTM（钻石） C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.5%磷酸溶液（10：90），流速1.0 mL·min-1，柱温40 ℃，检测波长280 nm。结果：绿原酸线性范围0.08~0.76 μg（r=0.999 9），平均加样回收率99.48%，RSD 2.82%。咖啡酸线性范围0.051~0.459 μg（r=0.999 9），平均加样回收率100.31%，RSD 0.96%。结论：该法简单、准确、重复性好，可用于测定短星火绒草中绿原酸和咖啡酸含量。

Abstract

Objective:To establish high performance liquid chromatography (HPLC) method for the determination of chlorogenic acid and caffeic acid in Leontopodium brachyactis from Xinjiang for the first time. Method:The stationary phase was DiamonsilTM C18 (4.6 mm×250 mm

5 μm) column and the mobile phase was acetonitrile-0.5% phosphoric acid (10:90). The flow rate was maintained at 1.0 mL·min-1 and the column temperature was set at 40 ℃. The detective wavelength was set at 280 nm. Result:The liner range of chlorogenic acid was 0.08-0.76 μg (r=0.999 9). The average recovery was 99.48% (RSD 2.82%). The liner range of caffeic acid was 0.051-0.459 μg (r=0.999 9). The average recovery was 100.31% (RSD 0.96%). Conclusion:The method is simple

accurate and sensible

and can be used to determine the content of the chlorogenic acid and caffeic acid in L. brachyactis.

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