WEI Gui-lin, LI Ze-lin, WU Jun, et al. RP-HPLC with UV Switch Determination of Three Active Components in Fukang Tablet[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 58-61.
DOI:
WEI Gui-lin, LI Ze-lin, WU Jun, et al. RP-HPLC with UV Switch Determination of Three Active Components in Fukang Tablet[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 58-61. DOI: 10.13422/j.cnki.syfjx.2014110058.
RP-HPLC with UV Switch Determination of Three Active Components in Fukang Tablet
Objective:To establish a simultaneous determination method for paeoniflorin
tetrahydropalmatine and ferulic acid in Fukang tablet. Method:The determination was performed on a Sepax C18 column with acetonitrile-0.1% ammoniae aqua as the mobile phase. The detection wavelength were at 230 nm (0-16 min
paeoniflorin)
280 nm (16.1-25 min
tetrahydropalmatine) and 320 nm (25.1-50 min
ferulic acid)
the flow rate was 1.0 mL·min-1. The column temperature was 30 ℃. Result:The paeoniflorin
tetrahydropalmatine and ferulic acid presented a good linearity in the concentration range of 0.242-2.42
0.02-0.203
0.045-0.448 μg (r≥0.999 6)
The average rate of recovery was 99.75%
100.33% and 100.45% with RSD=0.61%
1.46%
1.73% (n=6) respectively. Conclusion:This method was simple
accurate and reproducible
it can be used to determine the content of paeoniflorin
tetrahydropalmatine and ferulic acid in Fukang tablet.