Dynamic Changes of Rutin and Total Polysaccharides in  from Different Parts

CHEN Liang; CHEN Gang; XU Xiao-qin

doi:10.13422/j.cnki.syfjx.2014110066

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Dynamic Changes of Rutin and Total Polysaccharides in from Different Parts

更新时间：2024-01-04

- Dynamic Changes of Rutin and Total Polysaccharides in from Different Parts
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 11, Pages: 66-69(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014110066
  CLC： R284.1
- Published：2014
- 稿件说明：
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CHEN Liang, CHEN Gang, XU Xiao-qin. Dynamic Changes of Rutin and Total Polysaccharides in from Different Parts[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 66-69.
DOI：

CHEN Liang, CHEN Gang, XU Xiao-qin. Dynamic Changes of Rutin and Total Polysaccharides in from Different Parts[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 66-69. DOI： 10.13422/j.cnki.syfjx.2014110066.

摘要

目的：建立测定骆驼刺中总还原糖与芦丁含量的方法，并对不同部位的样品含量进行比较。方法：以硫酸蒽酮法，采用紫外分光光度法测定骆驼刺中总还原糖的含量，以葡萄糖为对照品，检测波长620 nm；采用高效液相色谱法测定芦丁的含量，色谱柱Symmetry Shield Rp-18（3.9 mm×150 mm，5 μm），流动相乙腈-0.1%磷酸水溶液（19：81），检测波长360 nm，柱温30 ℃。结果：葡萄糖检测的线性范围3.85~70.33 mg·L-1（r=0.995 9），平均加样回收率99.60%，RSD 1.5%（n=6）；芦丁检测的线性范围4.95~59.40 mg·L-1（r=0.999 6），平均加样回收率99.30%，RSD 2.08%。结论：不同部位的骆驼刺中芦丁和总还原糖含量有一定差异，其中果实及地上部分中总还原糖和芦丁的含量比较高。该结果可为骆驼刺的种植和采收的研究奠定基础。

Abstract

Objective:To establish the methods for determination of total polysaccharides and rutin content in Alhagi sparsifolin

and to compare the content of each sample from the different parts. Method:With glucose as control

total polysaccharides were measured by UV spectrophotometry at detection wavelength of 620 nm by anthrone sulphuric acid colorimetry. The content of rutin was determined by HPLC. The determination was performed on Symmetry shield Rp18 (3.9 mm×150 mm

5 μm) column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solvent (19:81) with the detection wavelength at 360 nm and the column temperature at 30 ℃. Result:The linear range of total polysaccharides was 3.85-70.33 mg·L-1(r=0.995 9)

and the linear range of rutin was 4.95-59.4 mg·L-1(r=0.999 6). Total polysaccharides and rutin in A. sparsifolin from different parts had certain differences. Conclusion:A. sparsifolin cultivated in the fruit and in part on the ground harvest in the total polysaccharides and the content of rutin are higher. It will provide foundation for the research for A. sparsifolin cultivation and harvest.

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Related Institution

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