Analysis of Monosaccharide Compositions of Polysaccharides in Coptidis Rhizoma by Pre-column Derivatization HPLC Method

FAN Gang; TANG Ce; LI Yan; YANG Yong-dong; ZHANG Yi

doi:10.13422/j.cnki.syfjx.2014110074

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Analysis of Monosaccharide Compositions of Polysaccharides in Coptidis Rhizoma by Pre-column Derivatization HPLC Method

更新时间：2024-01-04

- Analysis of Monosaccharide Compositions of Polysaccharides in Coptidis Rhizoma by Pre-column Derivatization HPLC Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 11, Pages: 74-78(2014)
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- DOI：10.13422/j.cnki.syfjx.2014110074
  CLC： R284.1
- Published：2014
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FAN Gang, TANG Ce, LI Yan, et al. Analysis of Monosaccharide Compositions of Polysaccharides in Coptidis Rhizoma by Pre-column Derivatization HPLC Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 74-78.
DOI：

FAN Gang, TANG Ce, LI Yan, et al. Analysis of Monosaccharide Compositions of Polysaccharides in Coptidis Rhizoma by Pre-column Derivatization HPLC Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 74-78. DOI： 10.13422/j.cnki.syfjx.2014110074.

摘要

目的：建立柱前衍生HPLC测定黄连多糖中的单糖组成，并分析3种黄连多糖的单糖差异。方法：采用水提醇沉法提取黄连多糖，经硫酸水解后，用1-苯基-3-甲基-5-吡唑啉酮（PMP）柱前衍生，再利用HPLC法分析单糖的PMP衍生物。结果：黄连多糖主要由甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖7种单糖组成，其中半乳糖醛酸含量最高，葡萄糖次之。另外，雅连多糖、云连多糖和味连多糖中的7种单糖的平均摩尔比分别为1：2.34：0.38：17.58：12.40：6.11：5.93，1：2.43：0.25：16.76：15.18：6.51：9.78和1：3.25：0.40：22.35：12.96：6.66：10.28。结论：建立的方法简便、准确，重复性好，可用于黄连多糖中单糖的组成分析和含量测定。雅连多糖、云连多糖和味连多糖中的7种单糖含量有差异。

Abstract

Objective:To establish a pre-column derivation HPLC method for the determination of monosaccharide compositions in Coptidis Rhizoma polysaccharides

and analyze the monosaccharides differences between three Coptidis Rhizoma polysaccharides. Method:The polysaccharides were extracted by hot distilled water

precipitated by alcohol

and hydrolyzed into monosaccharides with 2 mol·L-1 sulfuric acid. The hydrolysate was derivatized with PMP

and then the PMP derivates of monosaccharides were analyzed by HPLC method. Result:The Rhizoma Coptidis polysaccharides were composed of mannose

rhamnose

glucuronic acid

galacturonic acid

glucose

galactose and arabinose. Among them

the content of galacturonic acid was the most abundant

followed by glucose. In addition

the average molar ratio of seven monosaccharides of C. deltoidea polysaccharide

C. teeta polysaccharide and C. chinensis polysaccharide were 1:2.34:0.38:17.58:12.40:6.11:5.93

1:2.43:0.25:16.76:15.18:6.51:9.78 and 1:3.25:0.40:22.35:12.96:6.66:10.28

respectively. Conclusion:The established method in this study is simple

accurate

reproducible

and can be used for the analysis of monosaccharide compositions of Coptidis Rhizoma polysaccharides. The content of seven monosaccharides of C. deltoidea polysaccharide

C. teeta polysaccharide and C. chinensis polysaccharide are different.

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