GC Fingerprint of Volatile Constituents of Chrysanthemi Indici Flos from Different Areas

SONG Qing-po; HAN Yong-cheng; CHEN Ning; LIU Wei; WU Ming-xia; CUI Yong-xia; HUANG Li-jie

doi:10.13422/j.cnki.syfjx.2014110079

您当前的位置：

首页 >

文章列表页 >

GC Fingerprint of Volatile Constituents of Chrysanthemi Indici Flos from Different Areas

更新时间：2024-01-04

- GC Fingerprint of Volatile Constituents of Chrysanthemi Indici Flos from Different Areas
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 11, Pages: 79-82(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014110079
  CLC： R284.1
- Published：2014
- 稿件说明：
移动端阅览
SONG Qing-po, HAN Yong-cheng, CHEN Ning, et al. GC Fingerprint of Volatile Constituents of Chrysanthemi Indici Flos from Different Areas[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 79-82.
DOI：

SONG Qing-po, HAN Yong-cheng, CHEN Ning, et al. GC Fingerprint of Volatile Constituents of Chrysanthemi Indici Flos from Different Areas[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 79-82. DOI： 10.13422/j.cnki.syfjx.2014110079.

摘要

目的：建立不同产地野菊花挥发油成分气相指纹图谱，为有效控制和科学评价野菊花药材质量提供依据。方法：采用气相色谱法，HP-5（0.25 μm×0.32 mm×30 m）柱；程序升温，初始温度60 ℃，保持2 min，以4 ℃·min-1升至105 ℃，以2 ℃·min-1升至115 ℃，以4 ℃·min-1升至150 ℃，以15 ℃·min-1升至250 ℃保持2 min；载气高纯氮气（≥99.999%），流量1.0 mL·min-1；分流比为20：1，检测器FID，检测器温度250 ℃，进样口温度250 ℃。结果：对12个产地的野菊花进行了分析，建立了野菊花挥发油成分气相色谱指纹图谱。S1，S2，S8的相似度在0.850以下，S3，S4，S7，S10的相似度在0.850~0.950之间，其他的样品的相似度在0.950以上。结论：建立了野菊花挥发油成分气相指纹图谱方法，该方法重复性好，简单，易行，可为野菊花整体质量评价提供依据。

Abstract

Objective:To develop an GC method of determining the characteristic chromatographic profile of Chrysanthemi Indici Flos for controlling the drug quality quickly and accurately. Method:The separation was performed on HP-5 (0.25 μm×0.32 mm×30 m); the column oven was begun at 60 ℃ and held 2 min

then programmed from 60 ℃ to 105 ℃ at 4 ℃·min-1

then programmed from 105 ℃ to 115 ℃ at 2 ℃·min-1

then programmed from 115 ℃ to 250 ℃ at 15 ℃·min-1 held 2 min; by using helium as the carries gas

the flow rate is 1 mL·min-1

the split ratio is 20:1; hydrogen flame ionization detector; injector temperature was 250 ℃

detector temperature was 250 ℃. Result:To analyse 12 batches of volatile oil

the fingerprints of Chrysanthemi Indici Flos from different areas were established. The fingerprint similarity of S1

S8 is in 0.781-0.843.The fingerprint similarity of S3

S10 is in 0.853-0.932.The fingerprint similarity of the rest of the samples is above 0.95. Conclusion:This method is reproducible

simple and easy

and can be used to provide a base for the quality control of Chrysanthemi Indici Flos.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Simultaneous Determination of Chlorogenic Acid and Buddleoside in Chrysanthemi Indici Flos by UPLC

Qualitative and Quantitative Analysis of Chrysanthemi Indici Flos from Different Origins by Using NIRS and TQ Software

Rapid Determination of Buddleoside in Chrysanthemi Indici Flos of Henan Province by Near Infrared Spectroscopy

Anti-inflammatory and Analgesic Effects of Essential Oil from Chrysanthemi Indici Flos

Related Author

RU Fan-shu

WANG Li-guo

GONG Hai-yan

LEI Jing-wei

HAO Min

ZHANG Di-wen

BAI Yan

HU Xiao-li

Related Institution

No data

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰