Determination of Five Anthraquinone Derivatives in Wubei Yiwei Capsules by HPLC

WANG Qiang; QIAN Chun-hua; SUN Zhi-ming; WANG Jin-jin

doi:10.13422/j.cnki.syfjx.2014110095

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Determination of Five Anthraquinone Derivatives in Wubei Yiwei Capsules by HPLC

更新时间：2024-01-04

- Determination of Five Anthraquinone Derivatives in Wubei Yiwei Capsules by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 11, Pages: 95-98(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014110095
  CLC： R284.1
- Published：2014
- 稿件说明：
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WANG Qiang, QIAN Chun-hua, SUN Zhi-ming, et al. Determination of Five Anthraquinone Derivatives in Wubei Yiwei Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 95-98.
DOI：

WANG Qiang, QIAN Chun-hua, SUN Zhi-ming, et al. Determination of Five Anthraquinone Derivatives in Wubei Yiwei Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 95-98. DOI： 10.13422/j.cnki.syfjx.2014110095.

摘要

目的：建立测定乌贝益胃胶囊中5种蒽醌类衍生物（芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚）含量的方法。方法：采用高效液相色谱法，Eclipse XDB C18色谱柱（4.6 mm×150 mm，5 μm），流动相为甲醇-0.1%磷酸溶液（88：12），检测波长254 nm，柱温30 ℃，流速1.0 mL·min-1。结果：芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚5种成分进样量分别在0.067~0.335 μg（r=0.999 6），0.070~0.351 μg（r=0.999 8），0.068~0.341 μg（r=0.999 9），0.070~0.348 μg（r=0.999 9），0.038~0.191 μg（r=0.999 7）与各自峰面积积分值呈良好的线性关系，平均加样回收率分别为97.95%（RSD 0.98%），98.47%（RSD 1.18%），98.00%（RSD 0.71%），98.19%（RSD 0.68%），96.26%（RSD 1.35%）。结论：方法简便、准确、重复性好，可用于乌贝益胃胶囊的质量控制。

Abstract

Objective:To develop a high performance liquid chromatographic(HPLC) method for the determination of five anthraquinone derivatives (aloe-emodin

rhein

emodin

chrysophanol and physcion) in Wubei Yiwei capsules. Method:High performance liquid chromatography was perormed on an Eclipse XDB C18(4.6 mm×150 mm

5 μm)with methanol-0.1% phosphoric acid(88:12)as the mobile phase at a flow rate of 1.0 mL·min-1

254 nm as the detection wavelength

the temperature of column was at 30 ℃. Result:Five compounds showed good relationship at the range of 0.067-0.335 μg(r=0.999 6

for aloe-emodin)

0.070-0.351 μg(r=0.999 8

for rhein)

0.068-0.341 μg(r=0.999 9

for emodin)

0.070-0.348 μg(r=0.999 9

for chrysophanol)

0.038-0.191 μg(r=0.999 7

for physcion)

the average recoveries were 97.95%(RSD 0.98%)

98.47%(RSD 1.18%)

98.00%(RSD 0.71%)

98.19%(RSD 0.68%)

96.26%(RSD 1.35%). Conclusion:The results showed that the method is simple

accurate stable

and repeatable and it is suitable for quality control of Wubei Yiwei capsules.

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