Impact of Jiang Xinxia Formulae on Expression of HIF-1 and VEGF in Airway Remodeling of Asthmatic Rats

ZHANG Yu-ying; LUO Si-ning; YANG Jun; HE Peng; WANG Yan-feng

doi:10.13422/j.cnki.syfjx.2014110131

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Impact of Jiang Xinxia Formulae on Expression of HIF-1 and VEGF in Airway Remodeling of Asthmatic Rats

更新时间：2024-01-04

- Impact of Jiang Xinxia Formulae on Expression of HIF-1 and VEGF in Airway Remodeling of Asthmatic Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 11, Pages: 131-134(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014110131
  CLC： R285.5
- Published：2014
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ZHANG Yu-ying, LUO Si-ning, YANG Jun, et al. Impact of Jiang Xinxia Formulae on Expression of HIF-1 and VEGF in Airway Remodeling of Asthmatic Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 131-134.
DOI：

ZHANG Yu-ying, LUO Si-ning, YANG Jun, et al. Impact of Jiang Xinxia Formulae on Expression of HIF-1 and VEGF in Airway Remodeling of Asthmatic Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 131-134. DOI： 10.13422/j.cnki.syfjx.2014110131.

摘要

目的：研究姜辛夏方对OVA（卵蛋白）致敏哮喘大鼠模型肺泡灌洗液中缺氧诱导因子-1α（HIF-1α）及血管内皮生长因子（VEGF）表达的影响。方法：60只雄性SD大鼠分为正常对照组、哮喘模型组、姜辛夏方高、中、低剂量干预组（23，17.5，11.5 g·kg-1·d-1），甲泼尼龙干预组（0.76 mg·kg-1·d-1）。除正常对照组用生理盐水注射及气道雾化外，其余各组动物均以新鲜配制的卵蛋白抗原液做皮下、腹腔联合注射及气道雾化，建立大鼠哮喘模型。注射每天1次，连续5 d，实验第8天，各组于引喘前30 min灌胃给药干预，正常对照组、模型组用生理盐水，其余各干预组给予相应的药物灌胃进行干预。每隔1天用药引喘1次，连续30 d，观察整个实验过程中各组大鼠的一般情况，实验第39天采集标本，酶联免疫吸附法检测HIF-1α，VEGF的表达，并进行统计学分析。说明姜辛夏方对哮喘大鼠的疗效。结果：经过用抗原液引喘后，模型组大鼠烦躁不安、打喷嚏、抓鼻、喘息、倚笼而立、腹肌抽搐、大小便失禁，毛色暗淡，用药干预组与模型组比较症状明显减轻，正常组大鼠的表现未见明显异常。与正常组比较模型组肺泡灌洗液中HIF-1α、VEGF含量明显升高有显著差异（P<0.01），与模型组比较，姜辛夏方能明显减轻哮喘大鼠症状；明显降低肺泡灌洗液中HIF-1α及VEGF的含量（P<0.01），且姜辛夏方大剂量组的效果优于其他剂量组（P<0.05）。结论：姜辛夏方能抑制哮喘大鼠肺组织中HIF-1α及VEGF的生成，改善气道平滑肌与基底膜的增厚，从而抑制哮喘气道重建的发生发展。

Abstract

Objective:To study the impact of Jiang Xinxia formulae on the expression of hypoxia inducible factor 1α(HIF-1α) and vascular endothelia growth factor(VEGF) in the alveolar lavage fluid of egg albumin(OVA) induced asthmatic rats. Method:OVA was used to establish the of the rat asthmatic model. ELISA was used to measure the level of HIF-1α and VEGF in the alveolar lavage fluid. Result:Compared with model groups

Jiang Xinxia formulae obviously relieved the symptoms of the asthmatic rats; decreased the levelof the HIF-1α and VEGF in the alveolar lavage fluid(P<0.05). Conclusion:Jiang Xinxia formulae can inhibit the HIF-1α and VEGF in the asthmatic rat lungs

improve the thickening of the airway smooth muscle and basement membrane

thereby inhibit occurring and development of the asthmatic airway remodeling.

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