Effect of Amentoflavone on Inflammatory Factors in Rats with Acute Liver Injury

HUANG Zhen-qing; WEI Yan-fei; LIU Xue-mei; DUAN Xue-lin; ZHAO Tie-jian

doi:10.13422/j.cnki.syfjx.2014110147

您当前的位置：

首页 >

文章列表页 >

Effect of Amentoflavone on Inflammatory Factors in Rats with Acute Liver Injury

更新时间：2024-01-04

- Effect of Amentoflavone on Inflammatory Factors in Rats with Acute Liver Injury
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 11, Pages: 147-150(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014110147
  CLC： R285.5
- Published：2014
- 稿件说明：
移动端阅览
HUANG Zhen-qing, WEI Yan-fei, LIU Xue-mei, et al. Effect of Amentoflavone on Inflammatory Factors in Rats with Acute Liver Injury[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 147-150.
DOI：

HUANG Zhen-qing, WEI Yan-fei, LIU Xue-mei, et al. Effect of Amentoflavone on Inflammatory Factors in Rats with Acute Liver Injury[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 147-150. DOI： 10.13422/j.cnki.syfjx.2014110147.

摘要

目的：研究穗花杉双黄酮（ATF）对四氯化碳（CCl4）诱导急性肝损伤大鼠炎症相关因子的影响。方法：将健康雄性SD大鼠随机分为正常对照组，损伤模型组，阳性对照组，穗花杉双黄酮低、中、高剂量组（15，30，60 mg·kg-1），连续给药7 d，末次灌胃给药1 h后，腹腔注射CCl4原液建立急性肝损伤大鼠模型，采用ELISA法分别检测各组大鼠血清肿瘤坏死因子-α（TNF-α），转化生长因子β1 （TGF-β1），白介素1β（IL-1β），白细胞介素6（IL-6）的含量和肝组织核因子-κB （NF-κB），磷酸化抑制蛋白（nuclear factor kappa B inhibitor protein，IκB-α）的水平，采用鲎试剂终点显色法检测大鼠血浆内毒素（LPS）水平，Real time PCR法检测肝组织TNF-α和诱导型一氧化氮合酶（iNOS）基因的表达。结果：与正常组比较，模型组大鼠血清TNF-α，TGF-β1，IL-1β，IL-6的含量显著升高（P<0.01），大鼠血浆内毒素水平明显提高（P<0.01），肝组织NF-kB和磷酸化IκB-α的水平也明显升高（P<0.01），肝组织TNF-α和iNOS基因的表达也显著上调（P<0.01）。与模型组比较，ATF能显著降低血清TNF-α，TGF-β1，IL-1β，IL-6的含量（P<0.05 或P<0.01），降低肝组织NF-κB和磷酸化IκB-α的水平，且能显著降低LPS水平，下调肝组织TNF-α和iNOS基因的表达（P<0.05或P<0.01）。结论：穗花杉双黄酮对四氯化碳致急性肝损伤大鼠具有明显的保护作用，其机制可能通过抑制内毒素引起的Kupffer细胞激活，进而抑制下游核因子NF-κB的激活，减少炎症细胞因子的释放。

Abstract

Objective:To investigate the effects of amentoflavone (ATF) on inflammatory factors in rats with acute liver injury. Method:The healthy adult male Sprague-Dawley(SD) rats were randomly divided into six groups

control group

model group

positive control group

ATF low-dose

middle-dose and high-dose group(15

60 mg·kg-1)

respectively. The experimental rats were administrated with drugs by ig for 7 days

one hours after the last administration

the experimental rats were injected carbon tetrachloride to copy the acute liver injury model except control group. The contents of tumor necrosis factor-α(TNF-α)

transforming growth factor beta(TGF-β1)

interleukin 1β(IL-1β)

interleukin-6(IL-6) were investigated by ELISA. The phosphor-IκB-α level and nuclear factor κB(NF-κB) nuclear translocation in hepatic tissue were measured by ELISA. The plasma endotoxin(LPS) level was measured by limulus amebocyte lysate(LAL)chromogenic endpoint assay. The expression of TNF-α mRNA and induced nitric oxide synthase(iNOS) mRNA in hepatical tissues in rats were detected by Real time PCR. Result:Compared with the normal control group

the contents of TNF-α

TGF-β1

IL-1β and IL-6 in the serum of the model group rats were obviously increased(P<0.01)

and the level of plasma endotoxin was obviously increased(P<0.01)

the phosphor-IκB-α level and NF-κB nuclear translocation were obviously increased

the expression of TNF-α mRNA and iNOS mRNA were up-regulated(both P<0.01). Compared with the model group

the contents of TNF-α

TGF-β1

IL-1β

IL-6 were obviously decreased(P<0.05 or P<0.01)

The phosphor-IκB-α level and NF-κB nuclear translocation were decreased(P<0.05 or P<0.01)

and the level of plasma endotoxin was obviously decreased

the expression of TNF-α mRNA and iNOS mRNA in hepatical tissues in rats were down-regulated(P<0.05 or P<0.01). Conclusion:ATF could protect the acute hepatic injury induced by CCl4 in rats

the mechanism may be related to decreasing endotoxin level and NF-κB nuclear translocation and attenuating the trigger of inflammation-related cascade amplification.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Clinical Efficacy of Early Application of Da Xianxiongtang in Mitigating Progression of Acute Pancreatitis

Efficacy of Shexiang Tongxin Dropping Pills in Treatment of Coronary Slow Flow and Its Effect on Inflammatory Factors

Effect of Zishen Tongguan Formula on "Gut-prostate" Axis of Rats with Chronic Non-bacterial Prostatitis Based on 16S rDNA Sequencing

Effect of Rhei Radix et Rhizoma Before and After Steaming with Wine on Intestinal Flora and Immune Environment in Constipation Model Mice

Exploring Mechanism of Different Processed Products of Baiyaojian and Its Formula in Treating Ulcerative Colitis Based on Serum Metabolomics and Flow Cytometry

Related Author

REN Dongsheng

TAN Judan

LIAO Haitang

LIU Qinghai

WENG Xiangwen

GUO Wenhui

WANG Yiyan

ZHOU Yuling

Related Institution

Chengdu University of Traditional Chinese Medicine（TCM）

Chongqing TCM Hospital

Hospital of Chengdu University of TCM

Dongzhimen Hospital， Beijing University of Chinese Medicine

Jiangsu Engineering Research Centre for Innovative Chinese Medicine Preparations， Jiangsu Integration Innovation Centre for Specialty Chinese Medicine Preparations，Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Province Hospital of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰