Effect of Fuzhi Capsule on Long-term Potentiation of Hippocampus Neuronal Cell of Rats with Vascular Dementia

ZHANG Jie; MA Yun-zhi; ZHU Xiao-bin; YANG Ze-feng; LIU Zheng-wei

doi:10.13422/j.cnki.syfjx.2014110155

您当前的位置：

首页 >

文章列表页 >

Effect of Fuzhi Capsule on Long-term Potentiation of Hippocampus Neuronal Cell of Rats with Vascular Dementia

更新时间：2024-01-04

- Effect of Fuzhi Capsule on Long-term Potentiation of Hippocampus Neuronal Cell of Rats with Vascular Dementia
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 11, Pages: 155-159(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014110155
  CLC： R285.5
- Published：2014
- 稿件说明：
移动端阅览
ZHANG Jie, MA Yun-zhi, ZHU Xiao-bin, et al. Effect of Fuzhi Capsule on Long-term Potentiation of Hippocampus Neuronal Cell of Rats with Vascular Dementia[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 155-159.
DOI：

ZHANG Jie, MA Yun-zhi, ZHU Xiao-bin, et al. Effect of Fuzhi Capsule on Long-term Potentiation of Hippocampus Neuronal Cell of Rats with Vascular Dementia[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 155-159. DOI： 10.13422/j.cnki.syfjx.2014110155.

摘要

目的：观察复智胶囊对血管性痴呆大鼠海马神经元突触传递长时程增强（long-term potentiation，LTP）的影响。方法：SPF级雄性SD大鼠采用双侧颈总动脉永久结扎法（2-VO）制备血管性痴呆大鼠模型。选取造模成功的大鼠，随机分为模型组、多奈哌齐（0.52×10-3 g·kg-1·d-1，ig）组、复智胶囊高、低剂量（6.30，3.15 g·kg-1·d-1，ig）组。并设假手术对照组。连续ig 4周后，采用Morris水迷宫检测实验大鼠的学习记忆能力，同时采用LTP诱导法检测大鼠海马齿状回高频刺激诱发的群峰电位（population spike，PS）幅值变化，采用免疫组化检测海马N-甲基-D-天门冬氨酸（NMDA）免疫阳性细胞的表达。结果：与假手术组相比，造模术后的大鼠学习记忆能力均有下降，逃避潜伏期和游泳路程明显延长，撤除平台后跨越原平台次数明显减少，高频电刺激后海马神经元齿状回PS增幅明显下降，海马NMDA免疫阳性细胞数明显减少，差异均有统计学意义（P<0.05）；而与模型组相比，复智胶囊高、低剂量组和多奈哌齐组大鼠学习记忆能力均有改善，逃避潜伏期和游泳路程均有缩短，跨越原平台次数明显增多，海马神经元齿状回PS增幅明显升高，海马NMDA免疫阳性细胞数明显增多，差异均有统计学意义（P<0.05）。结论：复智胶囊通过增加海马NMDA阳性细胞表达，提高海马神经元齿状回PS增幅，改善大鼠学习记忆能力，对血管性痴呆模型大鼠海马神经元突触传递长时程增强有明显的改善作用。

Abstract

Objective:To observe the Fuzhi Capsule on long-term potentiation of hippocampus neuronal cell of rats with vascular dementia. Method:The SPF male SD rats were divided into model group

Donepezil group

Fuzhi capsule high and low dosage groups

and sham operation control group randomly.The vascular dementia model was established by permanent bilateral common carotid artery ligation (2-VO). Drugs were administered to rats by gastric perfusion once a day for 4 weeks. Then all the rats were detected the behavior by Morris water maze

the changes of population spike potential amplitude induced by the high frequency stimulation will be detected by long-term potentiation(LTP) inducted method and the expression of N-niethyl-D-asportate(NMDA) in hippocampus by immunohistochemistry. Result:Compared with the sham operation group

the learning and memory ability of rats in model group were decreased

the escape latency and swimming distance were significantly prolonged

the number of across the platform after remove platform were decreased

the population spike potential amplitude of hippocampal neurons in dentate gyrus decreased markedly

the number of NMDA immunoreactive cells was significantly reduced(P<0.05). Compared with the model group

the learning and memory ability of rats in Fuzhi capsule high and low dosage group and donepezil group were improved

the escape latency and swimming distance were shortened

the number of across the platform after remove platform were increased obviously

the population spike potential amplitude of hippocampal neurons in dentate gyrus increased markedly

the number of NMDA immunoreactive cells was significantly increased(P<0.05). Conclusion:Fuzhi capsule can improve the learning and memory ability of rats with vascular dementia by increasing the number of NMDA immunoreactive cells and the population spike potential amplitude of hippocampal neurons

improve the long term potentiation of synapses of hippocampal neurons of rat with vascular dementia.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Fuzhi Capsule on Inflammation Factors and Acetylcholine System in Rats with Vascular Dementia

Effect of Huanglian Jiedutang in Improving Brain Iron Metabolism Disorders， Myelin Damage， and Aggressive Behavior in VaD Mice via Regulation of FBXL5/IRP2

Tongmai Kaiqiao Pills Treat Vascular Dementia in Rats by Regulating Mitochondrial Autophagy via HIF-1α/BNIP3 Signaling Pathway

Modified Shuyuwan Ameliorates Depressive Behaviors and Promotes Myelin Regeneration in Mouse Model of Vascular Dementia Complicated with Depression by Regulating Energy Metabolism of Myelin Axons via MCT1

Safety Evaluation of Tianzhi Granules in Treating Mild-to-moderate Vascular Dementia

Related Author

WANG Bao-qi

CHENG Chuan-hao

MA Yun-zhi

FENG Xiaoxia

CHEN Chen

QIU Jing

YANG Guang

DING Huimin

Related Institution

Hubei Shizhen Laboratory

Hubei Provincial Institute of TCM，Hubei Key Laboratory of Theory and Application Research of Liver and Kidney in TCM

Hubei Provincial Hospital of Traditional Chinese Medicine（TCM）

The First Clinical Medical School，Hubei University of Chinese Medicine

Henan Provincial Hospital of Traditional Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰