Applications of Affinity Chromatography in Natural Medicine Research

CHEN Fang-you; LUO Yong-ming; WU Yang-ming; JIANG Chen-xing; ZHANG Xue-lian

doi:10.13422/j.cnki.syfjx.2014110230

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Applications of Affinity Chromatography in Natural Medicine Research

更新时间：2024-01-04

- Applications of Affinity Chromatography in Natural Medicine Research
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 11, Pages: 230-234(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014110230
  CLC： R284.1
- Published：2014
- 稿件说明：
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CHEN Fang-you, LUO Yong-ming, WU Yang-ming, et al. Applications of Affinity Chromatography in Natural Medicine Research[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 230-234.
DOI：

CHEN Fang-you, LUO Yong-ming, WU Yang-ming, et al. Applications of Affinity Chromatography in Natural Medicine Research[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 230-234. DOI： 10.13422/j.cnki.syfjx.2014110230.

摘要

亲和色谱是利用生物分子与亲和色谱固定相表面配位体之间特效性亲和吸附作用来进行选择性分离生物分子的分离方法，具有高选择性、高活性回收率和高纯度等特点，广泛的用于分离、检测或研究复杂样品中的目标分子，已逐渐成为天然药物研究中的重要手段。本文概述了免疫亲和色谱、凝集素亲和色谱、细胞膜色谱法、固定化金属亲和色谱、分子烙印色谱、染料配基亲和色谱、核酸适配体亲和色谱、亲和膜色谱、前沿亲和色谱等几种常用亲和色谱技术的配基的种类和选择方法，重点介绍了它们在天然药物研究中的应用进展，并对其应用前景进行了展望。

Abstract

Affinity chromatography is a method using the specific affinity adsorption between biological molecules and surface-ligand of affinity chromatography stationary phase for selective separation of biomolecules.It has been widely used for separation

detection or study among the target molecules of complex samples with the advantages of high selective

high recovery efficiency and high purity.Affinity chromatography has become a very important method in natural medicine research gradually. The commonly used categories consist of immunoaffinity chromatography

lectin affinity chromatography

cell membrane chromatography

immobilized metal affinity chromatography

molecular imprinting

dye ligands affinity chromatography

aptamer affinity chromatography

affinity membrane chromatography and frontal affinity chromatography. The applications in natural medicine research of the main categories of affinity chromatography

the types and the preparations and choice methods of ligands are introduced in this review

and its application is prospected as well.

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