Optimization of Extraction Process for Total Alkaloids from  by Ultrasonic Assisted Aqueous Two-phase System

GUO Tao; SUN Li; HUANG Yan; LV Guang-yao; ZHANG Bing-yun

doi:10.13422/j.cnki.syfjx.2014130008

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Optimization of Extraction Process for Total Alkaloids from by Ultrasonic Assisted Aqueous Two-phase System

更新时间：2024-01-04

- Optimization of Extraction Process for Total Alkaloids from by Ultrasonic Assisted Aqueous Two-phase System
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 13, Pages: 8-11(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014130008
  CLC： R284.2
- Published：2014
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GUO Tao, SUN Li, HUANG Yan, et al. Optimization of Extraction Process for Total Alkaloids from by Ultrasonic Assisted Aqueous Two-phase System[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 8-11.
DOI：

GUO Tao, SUN Li, HUANG Yan, et al. Optimization of Extraction Process for Total Alkaloids from by Ultrasonic Assisted Aqueous Two-phase System[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 8-11. DOI： 10.13422/j.cnki.syfjx.2014130008.

摘要

目的：优选超声波辅助双水相体系提取竹叶椒总生物碱的工艺条件。方法：采用超声波辅助丙醇-硫酸铵双水相体系提取竹叶椒总生物碱，选择提取时间、醇水质量比和盐用量为自变量，总生物碱提取率为因变量，通过三因素三水平的响应面试验优选提取工艺。结果：最佳提取工艺为提取时间40 min，醇水质量比0.5：1，盐用量7.79 g；总生物碱提取率1.704%，与模型预测值（1.711%）偏差很小，纯度19.8%。结论：超声波辅助双水相体系法提取总生物碱具有效率高、纯度高的特点，可避免不稳定或易变性的生物活性分子变性。

Abstract

Objective: To optimize ultrasonic assisted aqueous two-phase system extraction technology of total alkaloids from Zanthoxylum armatum. Method: Total alkaloids from Z. armatum was extracted by ultrasonic extraction technology coupling with propyl-alcohol ammonium sulfate aqueous two-phase system

taking extracting time

mass ratio of alcohol to water and salt dosage as independent variables

extraction rate of total alkaloids as dependent variable

a three response factors and three levels composite design of response surface methodology was employed to optimize extraction technology. Result: Optimal extraction process was as following:extracting time of 40 min

mass ratio of alcohol to water 0.5:1

salt dosage 7.79 g.Under these conditions

extraction rate of total alkaloids was up to 1.704%

which was agreed with model predicted value(1.711%)

purity of total alkaloids was 19.8%. Conclusion: Ultrasound assisted aqueous two-phase system for total alkaloids from Z. armatum had advantages of high extraction rate and high purity

which could avoid denaturation of bioactive molecules with instability or volatility.

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