Optimization of -Cyclodextrin Assisted Extraction Technology for Polygoni Multiflori Radix Praeparata by Multi-index Comprehensive Evaluation Method

CUI Ming-lei; REN Xiao-liang; LIU Ya-nan; QIU Xi-long; QI Ai-di

doi:10.13422/j.cnki.syfjx.2014130018

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Optimization of -Cyclodextrin Assisted Extraction Technology for Polygoni Multiflori Radix Praeparata by Multi-index Comprehensive Evaluation Method

更新时间：2024-01-04

- Optimization of -Cyclodextrin Assisted Extraction Technology for Polygoni Multiflori Radix Praeparata by Multi-index Comprehensive Evaluation Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 13, Pages: 18-21(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014130018
  CLC： R284.2
- Published：2014
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CUI Ming-lei, REN Xiao-liang, LIU Ya-nan, et al. Optimization of -Cyclodextrin Assisted Extraction Technology for Polygoni Multiflori Radix Praeparata by Multi-index Comprehensive Evaluation Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 18-21.
DOI：

CUI Ming-lei, REN Xiao-liang, LIU Ya-nan, et al. Optimization of -Cyclodextrin Assisted Extraction Technology for Polygoni Multiflori Radix Praeparata by Multi-index Comprehensive Evaluation Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 18-21. DOI： 10.13422/j.cnki.syfjx.2014130018.

摘要

目的：优选制何首乌的β-环糊精（β-CD）辅助提取工艺。方法：采用层次分析法，以二苯乙烯苷、大黄素及大黄素甲醚转移率的综合评分为指标，通过正交试验考察β-CD用量、料液比、提取次数及时间对制首乌有效成分提取工艺的影响。采用HPLC测定二苯乙烯苷、大黄素及大黄素甲醚含量，流动相乙腈（A）-0.1%磷酸水（B）梯度洗脱（0~5 min，15%~25%A；5~12 min，25%~30%A；12~15 min，30%~60%A；15~20 min，60%A；20~25 min，60%~75%A；25~31 min，75%~77%A），检测波长320（二苯乙烯苷），254 nm（大黄素、大黄素甲醚）。结果：最佳工艺条件为β-CD加入量10%，加10倍量水提取3次，每次1 h；二苯乙烯苷、大黄素及大黄素甲醚转移率分别为90.19%，8.39%，0.76%。结论：与传统水提法相比，β-CD辅助提取法能显著提高活性成分转移率。

Abstract

Objective: To optimize β-cyclodextrin(β-CD) assisted extraction process of Polygoni Multiflori Radix Praeparata. Method: Analytic hierarchy process was adopted

taking comprehensive score of transfer rates of emodin

physcion and stilbene glycoside as index

orthogonal test was applied to optimize extraction technology with solid-liquid ratio

β-CD dosage

extracting time and times as factors.HPLC was employed to determine contents of emodin

physcion and stilbene glycoside with mobile phase of acetonitrile(A)-0.1% phosphoric acid solution(B) for gradient elution(0-5 min

15%-25%A;5-12 min

25%-30%A;12-15 min

30%-60%A;15-20 min

60%A;20-25 min

60%-75%A;25-31 min

75%-77%A) and detection wavelength at 320(stilbene glycoside) and 254(emodin and physcion) nm. Result: Optimal extraction process was as following:extracted 3 times with 10-fold water for 1 h each time

β-CD dosage of 10%;Transfer rates of emodin

physcion and stilbene glycoside were 8.39%

0.76% and 90.19%

respectively. Conclusion: Compared with traditional water extraction

β-CD assisted extraction process could significantly improve transfer rate of active ingredients.

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