NIE Xiao-zhong, DANG Jian-zhang, WANG Xiao-li. Optimization of Separation and Purification Process for Praeruptorin B from Peucedani Radix with Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 35-37.
DOI:
NIE Xiao-zhong, DANG Jian-zhang, WANG Xiao-li. Optimization of Separation and Purification Process for Praeruptorin B from Peucedani Radix with Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 35-37. DOI: 10.13422/j.cnki.syfjx.2014130035.
Optimization of Separation and Purification Process for Praeruptorin B from Peucedani Radix with Macroporous Resin
Objective: To optimize purification process of praeruptorin B from Peucedani Radix by macroporous resin. Method: With extracting amount of praeruptorin B as index
orthogonal test was adopted to investigate effects of ethanol concentration
the amount of solvent and extracting time on extraction process.Type of macroporous resin and its elution conditions were investigated by single factor tests.HPLC was employed to determine the content of praeruptorin B with mobile phase of methanol-water(75:25) and detection wavelength at 321 nm. Result: Optimum extraction technology was as following:extracted 3.5 h with 8 times the amount of 60% ethanol.X-5 macroporous resin was selected
its adsorption time was 60 min
adsorption rate
elution rate
recovery and purity were 92.36%
71.24%
98.71% and 8.88%
respectively. Conclusion: X-5 macroporous resin was suitable for separation and purification of praeruptorin B
this study could provide a reference for development and applicaiton of total coumarins from Peucedani Radix.
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