DING Li-qin, JIN Jia-hua. Determination of Menthol, Camphor, Cineol and Eugenol in Essential Balm by GC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 85-89.
DOI:
DING Li-qin, JIN Jia-hua. Determination of Menthol, Camphor, Cineol and Eugenol in Essential Balm by GC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 85-89. DOI: 10.13422/j.cnki.syfjx.2014130085.
Determination of Menthol, Camphor, Cineol and Eugenol in Essential Balm by GC
Objective: To establish a GC method for determination of menthol
camphor
cineol and eugenol in essential balm. Method: Agilent HP-INNOWAX capillary column and FID detector were used. The temperature of the detector was 280 ℃
and the temperature of the entrance of the capillary vessel column was 250 ℃. The temperature programing rose from 90 ℃ to 250 ℃ by 10 ℃ per minute
lasting 10 min in the end. Result: Under the same chromatogram condition
there was linear relation among menthol
camphor
cineol and eugenol in the range of 0.028 6-2.86
0.028 52-2.852
0.015 784 2-1.578 42
0.004 386 8-0.438 68 μg
respectively. The average recovery rates were 99.17% (RSD 0.71%)
101.56% (RSD 0.80%)
104.22% (RSD 1.17%)
101.29% (RSD 1.17%)
respectively. Conclusion: The method is simple
accurate
reproducible
which can be applied to quality control of essential balm.