Intervention of Combined Different Particle of Fufang Xiyi San on Precancerous Lesions in Gastric Cancer Rats Via the Stat3 Pathway

LI Wei-qiang; ZHU Xi-jie; CAI Gen-shen

doi:10.13422/j.cnki.syfjx.2014130142

您当前的位置：

首页 >

文章列表页 >

Intervention of Combined Different Particle of Fufang Xiyi San on Precancerous Lesions in Gastric Cancer Rats Via the Stat3 Pathway

更新时间：2024-01-04

- Intervention of Combined Different Particle of Fufang Xiyi San on Precancerous Lesions in Gastric Cancer Rats Via the Stat3 Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 13, Pages: 142-146(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014130142
  CLC： R285.5
- Published：2014
- 稿件说明：
移动端阅览
LI Wei-qiang, ZHU Xi-jie, CAI Gen-shen. Intervention of Combined Different Particle of Fufang Xiyi San on Precancerous Lesions in Gastric Cancer Rats Via the Stat3 Pathway[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 142-146.
DOI：

LI Wei-qiang, ZHU Xi-jie, CAI Gen-shen. Intervention of Combined Different Particle of Fufang Xiyi San on Precancerous Lesions in Gastric Cancer Rats Via the Stat3 Pathway[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 142-146. DOI： 10.13422/j.cnki.syfjx.2014130142.

摘要

目的：研究复方蜥蜴散不同微粒组合剂对胃癌前病变（PLGC）信号传导与转录激活因子（Stat3）通路蛋白表达的影响，探讨其治疗PLGC机制。方法：将160只SPF级SD雄性大鼠随机分为空白对照组，复方蜥蜴散80目组（B，1.4 g·kg-1），100目组（C，1.4 g·kg-1），80目100目等量混合高、中、低剂量组（D，E，F，2.8，1.4，0.7 g·kg-1），模型对照组（E），维酶素组（H），除A外，均以0.017 mol·L-1 N-甲基-N’-硝基-N-亚硝基胍（MNNG）溶液，按5 mL·kg-1给大鼠ig，1次/d，连续24周，辅以饥饱失常，情绪刺激等制作PLGC大鼠模型。造模成功后，A组大鼠仍充足供食及清洁蒸馏水，B，C，D，E，F组大鼠分别给予复方蜥蜴散80目，100目，80目100目等量混合糊剂高、中、低剂量，混悬液10 mL·kg-1 ig，1次/d；G组大鼠给予0.9%氯化钠溶液10 mL·kg-1 ig，1次/d；H组大鼠给予维酶素混悬液（1.0 g·kg-1）ig，1次/d。连续12周。观察复方蜥蜴散对PLGC模型大鼠胃黏膜细胞Stat3，B细胞淋巴瘤/白血病2（Bcl-2）等抗凋亡蛋白影响，通过Image-Pro Plus 6.0图像分析系统对免疫组化图像的吸光度进行测算。结果：治疗前，各组与空白对照组相比，Stat3，Bcl-2阳性表达有显著差异（P<0.01），各组间无统计学差异。治疗后，各治疗组与模型组比较，Stat3，Bcl-2阳性表达有显著性差异（P<0.01），其中复方蜥蜴散各治疗组与维酶素组比较有差异（P<0.05），尤其以复方晰蜴散80目100目等量混合3个剂量组差异显著（P<0.01）。而复方蜥蜴散各治疗组间比较，B组与C组无统计学意义，而与D，E，F组比较则有差异（P<0.05），复方晰蜴散80目100目等量混合3个剂量组比较，D，E组与F组比较，P<0.05。结论：复方蜥蜴散不同微粒组合剂可逆转PLGC模型大鼠胃黏膜增生肠化，降低Stat3，Bcl-2阳性表达，调控细胞增殖和凋亡，且呈剂量相关性，这可能是复方蜥蜴散不同微粒组合剂干预PLGC的分子生物学机制。

Abstract

Objective: To study wether the intervention of the combined different particle of Fufang Xiyi San on precancerous lesions of gastric cancer(PLGC) in rat model is via the signal transducer and activator of transcription(Stat3) pathway. Method: One hundred and sixty SPF SD male rats were randomly divided into blank control group 1.4 g·kg-1

complex lizards powder 80 mesh treatment group 1.4 g·kg-1

100 mesh treatment group(2.8

1.4

0.7 g·kg-1)

and 80 mesh

100 mesh mixed high

medium and low dose treatment group

model control group

Weimeisu treatment group(1 g·kg-1)

in addition to the blank control group

0.017 mol·L-1 N-methyl-N'-nitro-N-nitrosogua nidine(MNNG) solution was given once daily

a combined method was used to establish PLGC model. After the 24 weeks

the corresponding drugs were given for 12 weeks. The antiapoptotic proteins Stat3 and B cell lymphoma/leukemia-2(Bcl-2) were measured. Result: Compared with model group after treatment

in each treatment group

Stat3 and Bcl-2 positive expression had a significant difference (P<0.01). Conclusion: The intervention of the combined different particle of fufang Xiyi san may be related to reducing the Stat3 and Bcl-2 positive expression.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Traditional Chinese Medicine Regulates Signaling Pathways Related to Precancerous Lesions of Gastric Cancer： A Review

Protective Effect of Qiling Prescription on Rats with Chronic Atrophic Gastritis Combined with Gastric Intestinal Metaplasia Via PI3K/Akt Pathway

Mechanism of Reversal of Precancerous Lesions of Gastric Cancer by Celastrus orbiculatus Extract via Regulating Lgr5/Wnt/β-catenin Signaling Pathway

Application of Theory of ''Pathogen Invading Nutrient and Blood Aspects'' in Precancerous Lesions of Gastric Cancer

Mechanism of NF-κB Signaling Pathway in Precancerous Lesions of Gastric Cancer and Treatment with Chinese and Western Medicine： A ReviewAI Yanan¹， ZHAO Weihan²

Related Author

ZHANG Zhiming

SONG Zhongyang

ZHAO Haihong

CHEN Lulu

ZHONG Jialin

LIU Yeyuan

SHEN Yanyun

LI Xinyu

Related Institution

Gansu Provincial Hospital of Traditional Chinese Medicine

Affiliated Hospital of Gansu University of Chinese Medicine

Gansu University of Chinese Medicine

Clinical College， Jiangsu Vocational College of Medicine

Lishui District Hospital of Traditional Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰