Acute Toxicity Test and Micronucleus Test of Water Extract from Cuscutae Semen in Rats

XIA Hui-fang; LI Xiao-hong

doi:10.13422/j.cnki.syfjx.2014130185

您当前的位置：

首页 >

文章列表页 >

Acute Toxicity Test and Micronucleus Test of Water Extract from Cuscutae Semen in Rats

更新时间：2024-01-04

- Acute Toxicity Test and Micronucleus Test of Water Extract from Cuscutae Semen in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 13, Pages: 185-188(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014130185
  CLC： R285.1
- Published：2014
- 稿件说明：
移动端阅览
XIA Hui-fang, LI Xiao-hong. Acute Toxicity Test and Micronucleus Test of Water Extract from Cuscutae Semen in Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 185-188.
DOI：

XIA Hui-fang, LI Xiao-hong. Acute Toxicity Test and Micronucleus Test of Water Extract from Cuscutae Semen in Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 185-188. DOI： 10.13422/j.cnki.syfjx.2014130185.

摘要

目的：探讨菟丝子水提液对SD大鼠的最大给药量的急性毒性与遗传毒性。方法：最大给药量法检测菟丝子水提液对SD大鼠的急性毒性。SD孕鼠骨髓微核试验及胚胎肝转移微核试验，设5组：菟丝子水提液高、中、低剂量组（40，20，10 g·kg-1）及阴性对照组（NS，10 mL·kg-1），阳性对照组[环磷酰胺（CP）40 mg·kg-1]，检测孕鼠骨髓嗜多染红细胞微核率及胚胎肝嗜多染红细胞微核率。结果：菟丝子水提液ig对SD大鼠的最大给药量为80 g·kg-1。菟丝子水提液40 g·kg-1可致孕鼠骨髓细胞、胚胎肝细胞嗜多染红细胞微核率升高，与阴性对照组对比，有显著性差异（P<0.01）。结论：菟丝子水提液对SD大鼠ig最大给药量为80 g·kg-1，属无毒级。菟丝子水提液ig剂量在40 g·kg-1时可诱发孕鼠及胚胎微核率增加，有潜在遗传毒性；而在20 g·kg-1以下剂量时无显著遗传毒性。

Abstract

Objective: To study the acute toxicity and inheritance toxicity of water extract of Cuscutae Semen in SD rats. Method: The acute toxicity of water extract of Cuscutae Semen in SD rats was detected by maximum tolerated dose method. Micronucleus test of pregnant mice bone narrow cells and fetal mice hepatocytes were carried out:5 groups were used for bone narrow and fetal hepatic cell

include high

middle and low dosage of water extract of Cuscutae Semen (40

10 g·kg-1

respectively)

negative control group (normal sodium

10 mL·kg-1) and positive control group

the micronucleus rates of bone marrow and fetal hepatic cell were evaluated by micronucleus tests. Result: The maximum dosage of administration of water extract Cuscutae Semen was 80 g·kg-1. Compared with negative control group

the micronucleus rates of bone marrow and fetal hepatic cell were all significantly increased (P<0.01) at a high dose of 40 g·kg-1. Conclusion: The maximum tolerance dose of water extract of Cuscutae Semen (80 g·kg-1) in SD rats is belonged to the non-toxic substance. The high dosage of water extract of Cuscutae Semen (40 g·kg-1) has potential genetic toxicity on micronuclear rates of pregnant mice bone marrow cell and fetal mice hepatocytes

but middle and low dosage of it (20

10 g·kg-1) have no genetic toxicity.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanism of Cuscutae Semen to Control Premature Ovarian Failure Based on Lipid and Atherosclerosis Pathways

Effect of Cuscutae Semen on Learning and Memory and N6-methyladenosine Modification in Hippocampus of Offspring of Fear-damaged Pregnant Rats

Protective Effect of Astragulus Polysaccharide on Chromosome Damage in Human BMSCs Exposed to Formaldehyde

Influence with Wine Processing on Content of Quercetin from Cuscutae Semen

Related Author

ZHAO Min

ZHANG Jiawei

SHI Ruoyi

ZHANG Jinrong

JIANG Xiaocui

XIAO Min

XIE Ying

LIU Yuexuan

Related Institution

School of Basic Medical Sciences， Hubei University of Chinese Medicine

Experimental Center of Traditional Chinese Medicine，Hubei University of Chinese Medicine

Maternal and Child Health Hospital of Hubei Province

Henan Province Engineering Technology Research Center for Chinese and Western Integrative Prevention and Treatment of Brain Cognitive Disease，Zhengzhou Key Laboratory of Chinese & Western Integrative Prevention and Treatment of Brain Cognitive Disease，Henan University of Chinese Medicine

Third Affiliated Hospital of Henan University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰