Optimization of Extraction Process of Ferritin from  by Response Surface Methodology

XIN Min; HUANG Yun; ZHAN Xin; WANG Ran; TANG Jin-tian

doi:10.13422/j.cnki.syfjx.2014140012

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Optimization of Extraction Process of Ferritin from by Response Surface Methodology

更新时间：2024-01-04

- Optimization of Extraction Process of Ferritin from by Response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 14, Pages: 12-16(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014140012
  CLC： TQ461;R284.2
- Published：2014
- 稿件说明：
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XIN Min, HUANG Yun, ZHAN Xin, et al. Optimization of Extraction Process of Ferritin from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 12-16.
DOI：

XIN Min, HUANG Yun, ZHAN Xin, et al. Optimization of Extraction Process of Ferritin from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 12-16. DOI： 10.13422/j.cnki.syfjx.2014140012.

摘要

目的：优选鹰嘴豆铁蛋白的提取工艺。方法：在单因素试验基础上，选择缓冲液pH、料液比、盐析盐摩尔浓度为自变量，干膏量及铁蛋白、总蛋白得率为响应值，根据Box-Behnken原理采用三因素三水平响应面分析法优选鹰嘴豆铁蛋白的提取工艺参数。结果：最佳提取工艺条件为缓冲液pH 7.46，料液比1：4，盐析盐摩尔浓度50 mmol·L-1；铁蛋白得率0.002 556%，与理论预测值0.002 633%偏差较小。结论：采用响应面法优选的鹰嘴豆铁蛋白提取工艺稳定合理，为提高铁蛋白得率和植物补铁制剂的开发利用提供参考。

Abstract

Objective: To optimize extraction process of ferritin from Cicer arietinum. Method: On the basis of single factor tests

response surface methodology(RSM) was applied for optimizing extraction conditions with solid-liquid ratio

buffer pH

salt concentration as independent variables and yields of ferritin and total proteins

dry weight of extract as response value.According to Box-Behnken principles

RSM with three factors and three levels was used. Result: Optimum extraction conditions for ferritin from C. arietinum was as follows:buffer pH of 7.46

solid-liquid ratio of 1:4

molar concentration of MgCl2 50 mmol·L-1;yield of ferritin was 0.002 556%

whose deviation was small with theoretical predicted value of 0.002 633%. Conclusion: RSM for optimizing extraction process of ferritin was stable and feasible

it could improve extraction conditions of ferritin and provide a reference for development and utilization of plant iron supplements.

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Related Institution

College of Pharmacy, Chengdu University of TCM

Key Laboratory of Active Ingredients in Traditional Chinese Medicine(TCM) Co-constructed by Jilin Province and Ministry of Education, Changchun University of Chinese Medicine

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