Objective: To investigate feasibility of UPLC-assisted for optimizing purification conditions of total flavonoids by macroporous resin. Method: Take aqueous extracts of Apocyni Veneti Folium as an example

taking content changes of total flavonoids

extract and main active components as indexes

on the basis on general elution conditions of flavonoids

UPLC was adopted to optimize elution conditions and experimental verification was employed.UV was used to determine the content of rutin with detection wavelength of 410 nm;contents of hyperin

isoquercetin and astragalin were determined by UPLC

mobile phase of acetonitrile-0.2% acetic acid (14:86)

flow rate of 0.2 mL·min-1

detection wavelength of 360 nm. Result: Optimum purification conditions were as follows:sample solution pH 5

diameter-high ratio 1:8

adsorption rate of 1.5 BV·h-1

standing for 2 h

washed impurity with 3 BV of water with elution rate of 1.5 BV·h-1

then eluted by 1 BV of 10% ethanol and 3 BV of 70% ethanol with elution rate of 2 BV·h-1

collected eluent of 70% ethanol;yield and purity of total flavonoids were 92.3% and 38.4%

respectively. Conclusion: Optimization of elution conditions by macroporous resin based on UPLC was simple and feasible with high accuracy.