HUANG Hui-lian, YAO Shun, HUANG Xiao-ying, et al. Identification of Metabolites of Resveratrol in Rat Urine and Bile Using LC-MS-MS[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 56-61.
DOI:
HUANG Hui-lian, YAO Shun, HUANG Xiao-ying, et al. Identification of Metabolites of Resveratrol in Rat Urine and Bile Using LC-MS-MS[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 56-61. DOI: 10.13422/j.cnki.syfjx.2014140056.
Identification of Metabolites of Resveratrol in Rat Urine and Bile Using LC-MS-MS
Objective: Identified the metabolites of resveratrol in vivo. Method: A method using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS-MS) analysis was established for the identification of metabolites of resveratrol. The rat urine and bile samples were pretreated by solid-phase extraction (SPE) method after oral administration at a dose of 100 mg·kg-1 of resveratrol. Result: Five metabolites were identified on their MS fragmentation behaviors by LC-MS-MS method with electrospray ionization (ESI) in negative ion mode. Conclusion: The results indicated the method could be used to rapid screen and identify the conceivable structures of active components responsible for pharmacological effects of resveratrol.
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