LUO Jun, QING Juan, ZHAO Lin-jun, et al. HPLC Fingerprint Chromatograms of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 69-72.
DOI:
LUO Jun, QING Juan, ZHAO Lin-jun, et al. HPLC Fingerprint Chromatograms of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 69-72. DOI: 10.13422/j.cnki.syfjx.2014140069.
Objective: To established the HPLC fingerprint chromatograms of Gentiana rhodantha. Method: The HPLC analysis was performed on a Diamonsil C18(4.6 mm×200 mm
5 μm) column with isocratic elution of acetonitrile-0.05% phosphoric acid at a flow rate of 0.8 mL·min-1.The detection wavelength was set at 245 nm
and the column temperature was 30℃. Result: The HPLC fingerprint chromatograms of G. rhodantha has been established
there were 40 common peaks
the similarities of the fingerprint chromatograms were calculated over 0.97 between 12 batches of G. rhodantha samples with mangiferin as reference substance. Conclusion: The method is repeatable
exclusive and can be used quality control of G. rhodantha.