LIU Hai-xia, PEI Xiang-ping, LI Hui-feng, et al. Determination of Content of Four Kinds of Fatty Acids in Seed by GC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 72-75.
DOI:
LIU Hai-xia, PEI Xiang-ping, LI Hui-feng, et al. Determination of Content of Four Kinds of Fatty Acids in Seed by GC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 72-75. DOI: 10.13422/j.cnki.syfjx.2014140072.
Determination of Content of Four Kinds of Fatty Acids in Seed by GC
Objective: To establish a method for the determination of the content of four kinds of fatty acids in Luffa cylindrica seed by GC
and provide evidence for evaluating quality and criterion. Method: The GC separation was performed on a HP-FFAP column(0.2 mm×50 m
0.3 μm) at 235℃
inject temperature at 250℃
FID detecter temperature was 250℃. Gas as N2(99.99%);the flow rate was 0.5 mL·min-1;split ratio 23:1. Result: The content of palmtinic acid in L. cylindrica seed were 2.325%-2.985%
octadecanoic acid 1.198%-1.771%
oleic acid 2.212%-5.159%
linoleic acid 7.383%-13.578%. Conclusion: The method can be used to control the quality of four kinds fatty acids in of L. cylindrica seed
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