Study of Binding and Molecular Docking Between Isorhamnetin-3--neohesperidoside and Bovine Serum Protein

DAI Guo-liang; MA Shi-tang; LIU Shi-jia; SUN Bing-ting; CHENG Xiao-gui; JU Wen-zheng; TAN Heng-shan

doi:10.13422/j.cnki.syfjx.2014140109

您当前的位置：

首页 >

文章列表页 >

Study of Binding and Molecular Docking Between Isorhamnetin-3--neohesperidoside and Bovine Serum Protein

更新时间：2024-01-04

- Study of Binding and Molecular Docking Between Isorhamnetin-3--neohesperidoside and Bovine Serum Protein
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 14, Pages: 109-113(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014140109
  CLC： R284.1
- Published：2014
- 稿件说明：
移动端阅览
DAI Guo-liang, MA Shi-tang, LIU Shi-jia, et al. Study of Binding and Molecular Docking Between Isorhamnetin-3--neohesperidoside and Bovine Serum Protein[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 109-113.
DOI：

DAI Guo-liang, MA Shi-tang, LIU Shi-jia, et al. Study of Binding and Molecular Docking Between Isorhamnetin-3--neohesperidoside and Bovine Serum Protein[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 109-113. DOI： 10.13422/j.cnki.syfjx.2014140109.

摘要

目的：研究异鼠李素-3-O-新橙皮糖苷与牛血清蛋白的相互作用。方法：采用平衡透析法，高效液相色谱测定异鼠李素-3-O-新橙皮糖苷与牛血清蛋白的结合率。采用同源建模和分子对接技术分析药物相互作用模式。结果：异鼠李素-3-O-新橙皮糖苷与牛血清蛋白的最佳平衡透析时间为16 h，异鼠李素-3-O-新橙皮糖苷低、中、高（2，4，8 mg·L-1）3个质量浓度的牛血清蛋白结合率分别为（43.23±2.23）%，（45.16±3.12）%，（44.71±3.25）%。牛血清白蛋白同源建模和分子对接结果显示：异鼠李素-3-O-新橙皮糖苷能够与牛血清白蛋白氨基酸残基形成6个氢键。结论：异鼠李素-3-O-新橙皮糖苷与牛血清发生中等强度的结合。

Abstract

Objective: To study the binding ratio of isorhamnetin-3-O-neohesperidoside with bovine serum protein in vitro. Method: Equilibrium dialysis and HPLC were employed to determine the binding rate of isorhamnetin-3-O-neohesperidoside and calf serum. And homology modeling and molecular docking were employed to simulate their interaction. Result: The balance time of dialysis was 16 hours approximately. The serum protein binding rate of isorhamnetin-3-O-neohesperidoside at low

middle and high concentrations (2

8 mg·L-1) were (43.23±2.23)%

(45.16±3.12)% and (44.71±3.25)%

respectively. The molecular docking showed that isorhamnetin-3-O-neohesperidoside was able to bind to residue of bovine serum albumin and formed six hydrogen bonds. Conclusion: Isorhamnetin-3-O-neohesperidoside has a medium extent of protein bind with bovine calf serum.

关键词

Keywords

references

Views

182

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mahoniae Caulis Alkaloids Ameliorate Depression by Regulating Synaptic Plasticity via cAMP Pathway

Analysis of Potential Active Components and Molecular Mechanism of Baoxin Granules Regulating Ferroptosis in Treatment of Heart Failure

Effect and Mechanism of Angelicae Sinensis Radix-Polygonati Rhizoma Herb Pair in Treatment of Simple Obesity

Analysis of Mechanism of Xingpi Capsules in Treatment of Functional Dyspepsia Based on Transcriptomics

Screening of Anti-breast Cancer Active Ingredients in Famous Classical Formula Yanghetang

Related Author

HE Junhui

JIA Chunlian

LAI Kedao

ZHOU Guili

ZHOU Rongfei

LI Yi

LI Dongmei

XIE Jiaxiu

Related Institution

Key Laboratory of Chemistry and Engineering of Forest Products， National Ethnic Affairs Commission， Guangxi Key Laboratory of Chemistry and Engineering of Forest Products/Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products， School of Chemistry and Chemical Engineering， Guangxi Minzu University

Guangxi Key Laboratory of Chinese Medicine Quality Standard， Guangxi Institute of Chinese Medicine and Pharmaceutical Science

Guangxi Medical University

State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs， Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences

Jiangxi Province Key Laboratory of Traditional Chinese Medicine（TCM） Pharmacology，Institute of TCM Health Industry，China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰