Activation Effect on Mushroom Tyrosinase of Seeds

LIU Ya-ya; WANG Qiang; PENG Yang

doi:10.13422/j.cnki.syfjx.2014140117

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Activation Effect on Mushroom Tyrosinase of Seeds

更新时间：2024-01-04

- Activation Effect on Mushroom Tyrosinase of Seeds
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 14, Pages: 117-119(2014)
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- DOI：10.13422/j.cnki.syfjx.2014140117
  CLC： R29
- Published：2014
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LIU Ya-ya, WANG Qiang, PENG Yang. Activation Effect on Mushroom Tyrosinase of Seeds[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 117-119.
DOI：

LIU Ya-ya, WANG Qiang, PENG Yang. Activation Effect on Mushroom Tyrosinase of Seeds[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 117-119. DOI： 10.13422/j.cnki.syfjx.2014140117.

摘要

目的：研究阿育魏实不同提取物对酪氨酸酶活性和黑色素生成的影响。方法：以阿育魏实为材料，通过乙醇提取得到总提取物即醇提物，然后依次用石油醚、三氯甲烷、乙酸乙酯、正丁醇萃取，得到相应的提取物。采用蘑菇酪氨酸酶多巴速率氧化法和NaOH裂解法研究了该提取物对酪氨酸酶的效应和黑色素上调率。结果：所得阿育魏实乙醇和乙酸乙酯提取物对酪氨酸酶激活作用较明显，对黑色素生成具有直接刺激作用，与对照品补骨脂和驱虫斑鸠菊相比，阿育魏实乙酸乙酯提取物IC50为（13.64±7.78） g·L-1，对酪氨酸酶激活作用优于临床治疗白癜风药物补骨脂[IC50 （21.18±2.88） g·L-1]。结论：阿育魏实乙酸乙酯提取物具有较好的体外酪氨酸酶激活及促进黑色素生成作用，可为临床治疗白癜风药物提供依据。

Abstract

Objective: The active effects on mushroom tyrosinase of the extracts from Trachyspermum ammi seeds and melanin synthesis were studied. Method: A ethanol extract of T. ammi was partitioned into petroleum ether

chloroform

ethyl acetate and n-butyl alcohol of the five fractions. Ethanol extract and ethyl acetate extract of T. ammi showed more potent in vitro activation of tyrosinase activity with L-DOPA as a substrate

and melanin content increasing activity. Result: The ethyl acetate extract of T. ammi activated tyrosinase at IC50 (13.64±7.78)g·L-1

and the IC50 value offructus psoraleae

a positive control

was (21.18±2.88)g·L-1. Conclusion: Ethyl acetate extract of T. ammi has a significant effect on activating the activity of tyrosinase and increasing melanin content in vitro. The project results showed an experimental evidence for clinical administration of the drugs in treating vitiligo.

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