Berberine Reduce TNF- and IL-6 Secreted in HUVEC Induced by Visfatin through ERK1/2 Signal Pathway

WAN Qiang; CUI Xiao-bing; JIA Yu-hua; ZHOU Feng-hua; ZHAO Xiao-shan; SUN Xue-gang; YANG Jing-hua

doi:10.13422/j.cnki.syfjx.2014140125

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Berberine Reduce TNF- and IL-6 Secreted in HUVEC Induced by Visfatin through ERK1/2 Signal Pathway

更新时间：2024-01-04

- Berberine Reduce TNF- and IL-6 Secreted in HUVEC Induced by Visfatin through ERK1/2 Signal Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 14, Pages: 125-129(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014140125
  CLC： R285
- Published：2014
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WAN Qiang, CUI Xiao-bing, JIA Yu-hua, et al. Berberine Reduce TNF- and IL-6 Secreted in HUVEC Induced by Visfatin through ERK1/2 Signal Pathway[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 125-129.
DOI：

WAN Qiang, CUI Xiao-bing, JIA Yu-hua, et al. Berberine Reduce TNF- and IL-6 Secreted in HUVEC Induced by Visfatin through ERK1/2 Signal Pathway[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(14): 125-129. DOI： 10.13422/j.cnki.syfjx.2014140125.

摘要

目的：观察细胞外调节蛋白激酶（ERK1/2）通路在内脏脂肪素（visfatin）诱导人脐静脉内皮细胞（HUVEC）分泌肿瘤坏死因子-α（TNF-α）及白介素-6（IL-6）中的作用及黄连素（berberine，Ber）的干预作用。方法：体外培养HUVEC，用visfatin不同质量浓度（0，50，100，200 μg·L-1）、不同时间（0，6，12，24，48 h）作用HUVEC，MTT法测HUVEC增殖，ELISA法测HUVEC上清液中TNF-α及IL-6含量；Western blotting法测HUVEC中p-ERK1/2蛋白表达；并用50 μmol·L-1 Ber及ERK1/2通路特异性抑制剂PD98059 20 μmol·L-1干预HUVEC，检测Ber的干预作用。结果：与对照组相比，100 μg·L-1 visfatin能显著抑制HUVEC增殖，升高HUVEC上清液中TNF-α及IL-6含量并能上调p-ERK1/2蛋白表达；与visfatin组比，Ber及PD98059均能显著减轻visfatin对HUVEC增殖的抑制，减少visfatin诱导HUVEC上清液中TNF-α及IL-6的含量并能下调HUVEC中p-ERK1/2蛋白的表达。结论： Ber可通过抑制炎症因子TNF-α及IL-6的分泌，减轻visfatin诱导的HUVEC损伤，其机制可能与抑制ERK1/2通路的激活有关。

Abstract

Objective: To study the role of extra cellular regulated protein kinasses(ERK1/2) pathway in tumor necrosis factor-alpha(TNF-α)and interleukin-6(IL-6)secreted in human umbilical vein endothelial cell(HUVEC)induced by visfatin and the interventive effect of berberine. Method: HUVEC were cultured for the experiment

added concentration gradient group of visfatin(0

100

200 μg·L-1)and time gradient group of visfatin(0

48 h)in HUVEC

MTT assay was used to detect the proliferation of HUVEC

the contents of TNF-α and IL-6 in HUVEC supernatant were determined by ELISA assay

the expression of p-ERK1/2 in HUVEC lysate were determined by Western blotting. Berberine(50 μmol·L-1)and PD98059 (20 μmol·L-1)

the specific inhibitor of ERK1/2 pathway

were added into HUVEC to detected the interventive effect of berberine. Result: Compared with the control group

visfatin(100 μg·L-1)can significantly decrease the proliferation of HUVEC and increase the TNF-α and IL-6 contents in HUVEC supernatant. Meanwhile

it can increase p-ERK1/2 expression at 24 hours. Compared with the visfatin group

berberine and PD98059 could increase the proliferation of HUVEC and decrease the TNF-α and IL-6 contents in HUVEC supernatant

at the same time

it could restrain p-ERK1/2 expression in HUVEC. Conclusion: Berberine can decrease the contents of TNF-α and IL-6 in HUVEC cultural supernatant and the injury induced by visfatin

the protective mechanism is related to the inhibition of ERK1/2 signal pathway activation.

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