Optimization of Ethanol Extraction Technology for Total Flavonoids in Roots of  and Screening of Its Type of Macroporous Resin

CHEN Yan; Wuliya·; Shayiti; MA Liang-ying

doi:10.13422/j.cnki.syfjx.2014150019

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Optimization of Ethanol Extraction Technology for Total Flavonoids in Roots of and Screening of Its Type of Macroporous Resin

更新时间：2024-01-04

- Optimization of Ethanol Extraction Technology for Total Flavonoids in Roots of and Screening of Its Type of Macroporous Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 15, Pages: 19-21(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014150019
  CLC： R284.2
- Published：2014
- 稿件说明：
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CHEN Yan, Wuliya·, Shayiti, et al. Optimization of Ethanol Extraction Technology for Total Flavonoids in Roots of and Screening of Its Type of Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 19-21.
DOI：

CHEN Yan, Wuliya·, Shayiti, et al. Optimization of Ethanol Extraction Technology for Total Flavonoids in Roots of and Screening of Its Type of Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 19-21. DOI： 10.13422/j.cnki.syfjx.2014150019.

摘要

目的：优选欧芹根总黄酮的提取工艺并筛选适合其富集纯化的大孔树脂型号。方法：采用UV测定总黄酮含量，检测波长510 nm。以总黄酮提取率和浸膏得率的综合评分为指标，通过正交试验考察提取时间、提取次数、乙醇体积分数及乙醇用量对欧芹根总黄酮提取工艺的影响；采用静态吸附-洗脱试验优选适合富集欧芹根总黄酮的大孔树脂型号。结果：最佳提取工艺条件为加6倍量70%乙醇提取2次，每次1 h；总黄酮提取率和浸膏得率分别约0.75%，14%。欧芹根总黄酮精制工艺中分离纯化效果最好的大孔吸附树脂为D101型。结论：优选的提取工艺稳定可行，筛选的大孔树脂分离纯化效果良好，为欧芹根的药物开发提供参考。

Abstract

Objective: To optimize extraction technology of total flavonoids in roots of Petroselinum crispum and screen type of macroporous resin which appropriated to its enrichment and purification. Method: UV was adopted to determine the content of total flavonoids with detection wavelength at 510 nm.With composite score of yields of total flavonoids and extract as index

orthogonal test was used to optimize extraction process by taking ethanol concentration

ratio of solid to liquid

extracting time and times as factors;the best type of macroporous resin was selected by static adsorption-elution test. Result: Optimum extraction process was as following:extracted twice with six times the amount of 70% ethanol for one hour per time;yields of total flavonoids and extract were 0.75% and 14%

respectively.D101 macroporous resin was the best one for purifying total flavonoids in roots of P. crispum. Conclusion: This optimized technology was stable and feasible

this screened resin had good separation and purification effect

this study could provide a reference for drug developmeng of roots of P. crispum.

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Related Institution

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Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

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