Objective: To optimize purification process of total coumarins from combined extract of Notopterygii Rhizoma et Radix and Angelicae Pubescentis Radix by macroporous resin. Method: UV was employed to determine the content of total coumarins with detection wavelength at 300 nm.Types of resin for seperation and purification of total coumarins were screened by static adsorption-elution test

taking the content of total coumarins as index

single factor tests were adopted to optimize purification conditions with the concentration of sample solution

sample volume

eluent concentration and amount as factors. Result: D101 macroporous resin had adsorption and disadsorption ability for total coumarins

optimum purification conditions were as follows:the concentration of sample solution 1.089 g·L-1

adsorption flow rate of 1 BV·h-1

sample volume of 2.5 BV

eluted with 4.5 BV of water and 2.5 BV of 80% ethanol;adsorption rate

desorption rate and purity of total coumarins were 91.03%

84.80% and 63.65%

respectively. Conclusion: Total coumarins from from combined extract of Notopterygii Rhizoma et Radix and Angelicae Pubescentis Radix could be purified effectively with D101 resin

this optimized process was simple

stable and feasible.